ArticlePLoS neglected tropical diseases2018
Both living bacteria and eukaryotes in the mosquito gut promote growth of larvae.
Article in PLoS neglected tropical diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 136 citations in OpenAlex.
- Crosstalk in the cold: host-microbe interactions in insect diapause.Annals of the Entomological Society of America · 2026Review
- Sterile Insect Technique: Investigating the Impact of Larval Diet and Ionizing Radiation on the Mosquito Bacterial Microbiota.Insects · 2026Article
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- Multi-Omics Profiling of the Scaphoideus titanus Yeast-Like Symbiont Guides the Bioinformatic Discovery of Related Fungal Symbioses in Insects.Environmental microbiology · 2026Article
- Flower- and Water-Dwelling Yeasts Influence Nectar-Seeking and Oviposition Behavior in the Asian Tiger Mosquito with Distinct Volatile Organic Compound Profiles.Journal of chemical ecology · 2026Article
- Transplant of cryopreserved mosquito gut microbiota reveals strain-specific patterns of microbial acquisition and assembly.ISME host microbe · 2026Article
- Transcriptional Control of Lipid Metabolism.Advances in experimental medicine and biology · 2026Review
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- Historical mosquito colonization dynamics are associated with patterns of microbial community assembly in aboveground aquatic habitats.Environmental microbiome · 2025Article
- Selected gut bacteria promote mosquito larval growth through the regulation of ionic microenvironment and protease expression.iScience · 2025Article
- Bacteria isolated from biodiverse Mediterranean island habitats yield a large array of biopesticidal metabolites against mosquito larvae.Applied and environmental microbiology · 2025Article
- Effect of trimethoprim on Calliphora stygia (Diptera: Calliphoridae) larvae growth and detection via liquid chromatography-tandem mass spectrometry.Journal of medical entomology · 2025Article
- Reduced microbe abundance in an urban larval development container increases Aedes aegypti susceptibility to Zika virus.PLoS pathogens · 2025Article
- Environmental yeasts differentially impact the development and oviposition behavior of the Asian tiger mosquito Aedes albopictus.Microbiome · 2025Article
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- Microbiome composition and turnover in the face of complex lifecycles and bottlenecks: insights through the study of dung beetles.Applied and environmental microbiology · 2025Article
- Microbial composition and function are nested and shaped by food web topologies.ISME communications · 2025Article
- Microbiota Composition Associates With Mosquito Productivity Outcomes in Belowground Larval Habitats.Molecular ecology · 2025Article
- Symbiotic Bacteria: Wolbachia, Midgut Microbiota in Mosquitoes and Their Importance for Vector Prevention Strategies.Microbial ecology · 2024Review
- MosAIC: An annotated collection of mosquito-associated bacteria with high-quality genome assemblies.PLoS biology · 2024Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
We recently reported that larval stage Aedes aegypti and several other species of mosquitoes grow when living bacteria are present in the gut but do not grow when living bacteria are absent. We further reported that living bacteria induce a hypoxia signal in the gut, which activates hypoxia-induced transcription factors and other processes larvae require for growth. In this study we assessed whether other types of organisms induce mosquito larvae to grow and asked if the density of non-living microbes or diet larvae are fed obviate the requirement for living organisms prior results indicated are required for growth. Using culture conditions identical to our own prior studies, we determined that inoculation density of living Escherichia coli positively affected growth rates of Ae. aegypti larvae, whereas non-living E. coli had no effect on growth across the same range of inoculation densities. A living yeast, alga, and insect cell line induced axenic Ae. aegypti first instars to grow, and stimulated similar levels of midgut hypoxia, HIF-α stabilization, and neutral lipid accumulation in the fat body as E. coli. However, the same organisms had no effect on larval growth if heat-killed. In addition, no axenic larvae molted when fed two other diets, when fed diets supplemented with heat-killed microbes or lysed and heat-killed microbes. Experiments conducted with An. gambiae yielded similar findings. Taken together, our results indicate that organisms from different prokaryotic and eukaryotic groups induce mosquito larvae to grow, whereas no conditions were identified that stimulated larvae to grow in the absence of living organisms.
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