Evidence map›Paper›PMID 29978422›Full record

ArticleParasitology research2018

DNA extraction from amoebal isolates and genotype determination of Acanthamoeba from tap water in Latvia.

Inese Gavarāne, Jūlija Trofimova, Artjoms Mališevs, Olga Valciņa, Muza Kirjušina, Ilze Rubeniņa, Aivars Bērziņš

Abstract read
PubMed Publisher
In one paragraph

Article in Parasitology research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Microbiology spectrum · 2026
    Article
  2. Immunopathogenicity ofInternational journal of molecular sciences · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Inese GavarāneInstitute of Food Safety, Animal Health and Environment BIOR, Lejupes Street 3, Riga, LV-1076, Latvia. inese.gavarane@du.lv.ORCID http://orcid.org/0000-0003-4927-0776
Jūlija TrofimovaInstitute of Food Safety, Animal Health and Environment BIOR, Lejupes Street 3, Riga, LV-1076, Latvia.
Artjoms MališevsInstitute of Food Safety, Animal Health and Environment BIOR, Lejupes Street 3, Riga, LV-1076, Latvia.
Olga ValciņaInstitute of Food Safety, Animal Health and Environment BIOR, Lejupes Street 3, Riga, LV-1076, Latvia.
Muza KirjušinaInstitute of Food Safety, Animal Health and Environment BIOR, Lejupes Street 3, Riga, LV-1076, Latvia.
Ilze RubeniņaInstitute of Food Safety, Animal Health and Environment BIOR, Lejupes Street 3, Riga, LV-1076, Latvia.
Aivars BērziņšInstitute of Food Safety, Animal Health and Environment BIOR, Lejupes Street 3, Riga, LV-1076, Latvia.
Institute of Food Safety, Animal Health and Environment “BIOR” · LVDaugavpils University · LV

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acanthamoeba are free-living amoebae found in various ecological environments, and they are major microbial predators. Some Acanthamoeba species can cause keratitis, granulomatous amoebic encephalitis, and infections of the organs. This is the first study in Latvia describing Acanthamoeba detection by molecular techniques and genotyping using tap water from different sources. An 18S rRNA gene investigation of the 20 Acanthamoeba isolates suggested that 19 belonged to the hazardous T4 genotype and one sample was T12. The results indicate that tap water in Latvia may be a source of Acanthamoeba that is potentially pathogenic to humans. All obtained sequences were submitted to the GenBank database under the following accession numbers: MG386295-MG386314.

Indexed as

AcanthamoebaAnimalsDatabases, Nucleic AcidDNA, ProtozoanGenotypeInvertebratesLatviaRNA, Ribosomal, 18SWaterWater PollutionDNA, ProtozoanRNA, Ribosomal, 18SWater18S rDNAAcanthamoebaLatviaTap water

Identifiers

PMID29978422
OpenAlexW2815343720

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.