ArticleJournal of veterinary research2017
Development of a Recombinant Protein-based ELISA for Detection of Antibodies Against Bovine Foamy Virus.
Article in Journal of veterinary research, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Molecular characterization of bovine foamy virus and its association with bovine leukemia virus infection in Vietnamese cattle.The Journal of veterinary medical science · 2021Article
- Article
- Genomic characterization and distribution of bovine foamy virus in Japan.The Journal of veterinary medical science · 2020Article
- Article
- Review
- Comparative evaluation of two commercial ELISA kits for detection of antibodies against Akabane virus in cattle serum.BMC veterinary research · 2019Article
- Isolation of an Equine Foamy Virus and Sero-Epidemiology of the Viral Infection in Horses in Japan.Viruses · 2019Article
- Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionInfections with bovine foamy virus (BFV) were found in many countries but there is a lack of large-scale surveys on the prevalence of BFV among dairy cattle. The aim of this study was to develop and validate the recombinant Gag protein-based ELISA and to estimate the prevalence of antibodies against BFV. MATERIAL AND
methodsGag coding region from BFV was cloned into expression vector pT7Arg-STOP, which expressed a high level of recombinant Gag protein from
resultsA total of 3,051 serum samples were tested by ELISA and 939 (30.8%) sera were recognised as positive. When Bayesian approach was used, the overall true BFV prevalence was 29.7% (95% CI: 25.9-33.4%).
conclusionExpressed Gag protein of BFV has been used successfully as an antigen for ELISA. Eventually, this study provides basic information about the epidemiological status of infection with BFV in dairy cattle in Poland, which can be used for further studies on dissemination and transmission of BFV infection.
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Registered trials
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