ArticleScientific reports2018
Visual Osteoclast Fusion via A Fluorescence Method.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Real-time analysis of osteoclast resorption and fusion dynamics in response to bone resorption inhibitors.Scientific reports · 2024Article
- Metal-polyDNA nanoparticles reconstruct osteoporotic microenvironment for enhanced osteoporosis treatment.Science advances · 2023Article
- Diterbutyl phthalate attenuates osteoarthritis in ACLT mice via suppressing ERK/c-fos/NFATc1 pathway, and subsequently inhibiting subchondral osteoclast fusion.Acta pharmacologica Sinica · 2022Article
- Aberrant NF-κB activation in odontoblasts orchestrates inflammatory matrix degradation and mineral resorption.International journal of oral science · 2022Article
- Fluorescence-Based Real-Time Analysis of Osteoclast Development.Frontiers in cell and developmental biology · 2021Article
- Monocyte/Macrophage Lineage Cells From Fetal Erythromyeloid Progenitors Orchestrate Bone Remodeling and Repair.Frontiers in cell and developmental biology · 2021Review
- Targeting actin-bundling protein L-plastin as an anabolic therapy for bone loss.Science advances · 2020Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Osteoclasts are multinucleated giant cells. Fusion is an essential element in the formation of osteoclasts. However, the exact cellular events and mechanisms remain largely unknown because of limited and insufficient methods for observing fusion process. In this work, a fluorescence reporter strategy was established to monitor osteoclast fusion. After fusing with cells expressing Cre recombinase, those cells with double fluorescence switch its expression from red to green fluorescent protein. The effect of RANKL and PTH on osteoclast fusion were both quantitatively and visually detected utilizing this strategy. Furthermore, a combination of this strategy with a technique of fluorescence-activated cell sorting revealed two different populations of fused osteoclasts, tdTomato+ GFP+ cells (TG cells) and GFP+ cells (G cells). The results argue for the potential of combining this technique with other bio-technologies to gain more information about osteoclast fusion. Overall, these data demonstrated that this visual fluorescence switch strategy is useful for further analysis of osteoclast fusion mechanisms.
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Registered trials
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