ArticleScientific reports2018
Reuse of malaria rapid diagnostic tests for amplicon deep sequencing to estimate Plasmodium falciparum transmission intensity in western Uganda.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- SIMPLseq: a high-sensitivity Plasmodium falciparum genotyping and PCR contamination tracking tool.Malaria journal · 2026Article
- Force of Infection (FOI) and Multiplicity of Infection (MOI) inPathogens (Basel, Switzerland) · 2024Article
- Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock.Frontiers in veterinary science · 2024Article
- Plasmodium falciparum population structure inferred by msp1 amplicon sequencing of parasites collected from febrile patients in Kenya.Malaria journal · 2023Article
- Comparing field-collected versus remotely-sensed variables to model malaria risk in the highlands of western Uganda.Malaria journal · 2023Article
- Comparative analysis of targeted next-generation sequencing for Plasmodium falciparum drug resistance markers.Scientific reports · 2022Article
- Occurrence and Distribution of Nonfalciparum Malaria Parasite Species Among Adolescents and Adults in Malawi.The Journal of infectious diseases · 2022Article
- Genetic surveillance for monitoring the impact of drug use on Plasmodium falciparum populations.International journal for parasitology. Drugs and drug resistance · 2021Review
- Next-generation sequencing of the soil nematode community enables the sustainability of banana plantations to be monitored.Applied soil ecology : a section of Agriculture, Ecosystems & Environment · 2021Article
- Malaria prevalence and long-lasting insecticidal net use in rural western Uganda: results of a cross-sectional survey conducted in an area of highly variable malaria transmission intensity.Malaria journal · 2021Article
- An open dataset ofWellcome open research · 2021Article
- Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests.Scientific reports · 2020Article
- A Targeted "Next-Generation" Sequencing-Informatic Approach to Define Genetic Diversity inPathogens (Basel, Switzerland) · 2020Article
- Elucidating cryptic dynamics ofEcology and evolution · 2020Article
- Strengthening Surveillance Systems for Malaria Elimination by Integrating Molecular and Genomic Data.Tropical medicine and infectious disease · 2019Review
- Prevalence of Molecular Markers of Antimalarial Drug Resistance across Altitudinal Transmission Zones in Highland Western Uganda.The American journal of tropical medicine and hygiene · 2019Article
- Proof of concept: used malaria rapid diagnostic tests applied for parallel sequencing for surveillance of molecular markers of anti-malarial resistance in Bissau, Guinea-Bissau during 2014-2017.Malaria journal · 2019Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Molecular techniques are not routinely employed for malaria surveillance, while cross-sectional, community-based parasite surveys require significant resources. Here, we describe a novel use of malaria rapid diagnostic tests (RDTs) collected at a single facility as source material for sequencing to esimtate malaria transmission intensity across a relatively large catchment area. We extracted Plasmodium falciparum DNA from RDTs, then amplified and sequenced a region of the apical membrane antigen 1 (pfama1) using targeted amplicon deep sequencing. We determined the multiplicity of infection (MOI) for each sample and examined associations with demographic, clinical, and spatial factors. We successfully genotyped 223 of 287 (77.7%) of the samples. We demonstrated an inverse relationship between the MOI and elevation with individuals presenting from the highest elevation villages harboring infections approximately half as complex as those from the lowest (MOI 1.85 vs. 3.51, AOR 0.25, 95% CI 0.09-0.65, p = 0.004). This study demonstrates the feasibility and validity of using routinely-collected RDTs for molecular surveillance of malaria and has real-world utility, especially as the cost of high-throughpout sequencing continues to decline.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.