ArticleNature protocols2018
High-throughput imaging flow cytometry by optofluidic time-stretch microscopy.
Article in Nature protocols, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 161 citations in OpenAlex.
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- Single-pixel imaging flow cytometry for biomedical research.Inflammation and regeneration · 2025Review
- Ultrahigh-throughput single-pixel complex-field microscopy with frequency-comb acousto-optic coherent encoding (FACE).Light, science & applications · 2025Article
- Spatial microfluidic holographic integrated platform for label-free and high-dimensional analysis of cancer heterogeneity.Nature communications · 2025Article
- Femtosecond laser microfabrication of a fully-integrated optofluidic device for 3D imaging flow cytometry.Scientific reports · 2025Article
- Imaging flow cytometry with a real-time throughput beyond 1,000,000 events per second.Light, science & applications · 2025Article
- Neuromorphic-enabled video-activated cell sorting.Nature communications · 2024Article
- Hyperdimensional computing: A fast, robust, and interpretable paradigm for biological data.PLoS computational biology · 2024Review
- Lab on a Particle Technologies.Analytical chemistry · 2024Review
- Raman micro-spectroscopy as a tool to study immunometabolism.Biochemical Society transactions · 2024Review
- DNA framework signal amplification platform-based high-throughput systemic immune monitoring.Signal transduction and targeted therapy · 2024Article
- Classification of fetal and adult red blood cells based on hydrodynamic deformation and deep video recognition.Biomedical microdevices · 2023Article
- Machine learning implementation strategy in imaging and impedance flow cytometry.Biomicrofluidics · 2023Review
- Visualisation of Host-Pathogen Communication.Advances in experimental medicine and biology · 2023Article
- Photonic Microfluidic Technologies for Phytoplankton Research.Biosensors · 2022Review
- The role of cellular traction forces in deciphering nuclear mechanics.Biomaterials research · 2022Review
Corrections and comments
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Authors and funding
15 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to rapidly assay morphological and intracellular molecular variations within large heterogeneous populations of cells is essential for understanding and exploiting cellular heterogeneity. Optofluidic time-stretch microscopy is a powerful method for meeting this goal, as it enables high-throughput imaging flow cytometry for large-scale single-cell analysis of various cell types ranging from human blood to algae, enabling a unique class of biological, medical, pharmaceutical, and green energy applications. Here, we describe how to perform high-throughput imaging flow cytometry by optofluidic time-stretch microscopy. Specifically, this protocol provides step-by-step instructions on how to build an optical time-stretch microscope and a cell-focusing microfluidic device for optofluidic time-stretch microscopy, use it for high-throughput single-cell image acquisition with sub-micrometer resolution at >10,000 cells per s, conduct image construction and enhancement, perform image analysis for large-scale single-cell analysis, and use computational tools such as compressive sensing and machine learning for handling the cellular 'big data'. Assuming all components are readily available, a research team of three to four members with an intermediate level of experience with optics, electronics, microfluidics, digital signal processing, and sample preparation can complete this protocol in a time frame of 1 month.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.