Evidence map›Paper›PMID 29976249›Full record

ArticleMicrobiome2018

Metagenomic analysis of basal ice from an Alaskan glacier.

Masood Ur Rehman Kayani, Shawn M Doyle, Naseer Sangwan, Guanqun Wang, Jack A Gilbert, Brent C Christner, Ting F Zhu

Abstract read
In one paragraph

Article in Microbiome, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Glaciers as microbial habitats: current knowledge and implication.Journal of microbiology (Seoul, Korea) · 2022
    Review
  3. Article
  4. Article
  5. Review
  6. Article
  7. Review
  8. HJournal of microbiology (Seoul, Korea) · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Masood Ur Rehman KayaniSchool of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Center for Synthetic and Systems Biology, Ministry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing, 100084, China.
Shawn M DoyleCollege of Geosciences, Texas A&M University, College Station, TX, 77843, USA.
Naseer SangwanBiosciences Division (BIO), Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL, 60439, USA.
Guanqun WangSchool of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Center for Synthetic and Systems Biology, Ministry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing, 100084, China.
Jack A GilbertBiosciences Division (BIO), Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL, 60439, USA. gilbertjack@uchicago.edu.
Brent C ChristnerDepartment of Microbiology and Cell Science, Biodiversity Institute, University of Florida, Gainesville, FL, 32611, USA. xner@ufl.edu.
Ting F ZhuSchool of Life Sciences, Tsinghua-Peking Joint Center for Life Sciences, Center for Synthetic and Systems Biology, Ministry of Education Key Laboratory of Bioinformatics, Tsinghua University, Beijing, 100084, China. tzhu@tsinghua.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundGlaciers cover ~ 10% of land but are among the least explored environments on Earth. The basal portion of glaciers often harbors unique aquatic microbial ecosystems in the absence of sunlight, and knowledge on the microbial community structures and their metabolic potential is very limited. Here, we provide insights into the microbial lifestyle present at the base of the Matanuska Glacier, Alaska.

resultsDNA and RNA were extracted from samples of the Matanuska Glacier basal ice. Using Illumina MiSeq and HiSeq sequencing, we investigated the microbial diversity with the metagenomic shotgun reads and 16S ribosomal RNA data. We further assembled 9 partial and draft bacterial genomes from the metagenomic assembly, and identified key metabolic pathways such as sulfur oxidation and nitrification. Collectively, our analyses suggest a prevalence of lithotrophic and heterotrophic metabolisms in the subglacial microbiome.

conclusionOur results present the first metagenomic assembly and bacterial draft genomes for a subglacial environment. These results extend our understanding of the chemical and biological processes in subglacial environments critically influenced by global climate change.

Indexed as

MetagenomicsAlaskaBacteriaBase SequenceBiodiversityEcosystemGenome, BacterialGeologic SedimentsIce CoverMicrobiotaNitrificationRNA, Ribosomal, 16SSequence Analysis, DNASulfurRNA, Ribosomal, 16SSulfurBasal ice layerGlacierMetagenomicsMicrobiome

Identifiers

PMID29976249
PMCPMC6034282

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.