ArticleBMC infectious diseases2018
Comparison of qPCR and culture methods for group B Streptococcus colonization detection in pregnant women: evaluation of a new qPCR assay.
Article in BMC infectious diseases, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers, 1 of them a synthesis that pooled it.
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Who cites it
27 citing papers in PubMed, 1 synthesis or guideline pooled it, 39 citations in OpenAlex.
- Rapid tests for detecting group B streptococcus colonization in pregnant women: A systematic review and meta-analysis of diagnostic accuracy.International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics · 2026Pooled it
- Iron Supplementation with Ferrous Sulfate or Ferrous Bisglycinate for 12 Weeks Does Not Influence Group B Streptococcus Colonization in Cambodian Women: A Secondary Analysis of a Randomized Controlled Trial.The Journal of nutrition · 2025Trial
- CAMP-negative group B Streptococcus in pregnant women: molecular and clinical features with implications for diagnostics and neonatal management.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- A novel real-time PCR assay for the simultaneous detection of the four main causes of bacterial meningitis.International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases · 2026Article
- Comparison of ESKAPE Pathogen Levels in Wastewater and Receiving Water Bodies Using Agar-Based Enumeration and Real-Time PCR.Current microbiology · 2026Article
- Article
- An improved procedure based on fluorescence immunochromatography for rapid detection of group B streptococcus from enrichment cultures.BMC pregnancy and childbirth · 2025Article
- Article
- Rapid Point-of-care Testing to Inform Intrapartum Treatment of Group BOpen forum infectious diseases · 2024Article
- Comparison of qPCR and chromogenic culture methods for rapid detection of group B streptococcus colonization in Vietnamese pregnant women.Practical laboratory medicine · 2024Article
- Development and performance evaluation of a qPCR-based assay for the fully automated detection of group BMicrobiology spectrum · 2024Article
- Adherence to universal screening for group B Streptococcus in pregnancy and prevalence of colonised pregnancies in Caserta province, Italy.Le infezioni in medicina · 2024Article
- Quantitative real-time PCR and magnetic separation strategy for specific detection of group B streptococcus in perinatal Women's urine.Practical laboratory medicine · 2024Article
- Establishment and application of a rapid molecular diagnostic platform for the isothermal visual amplification of group B Streptococcus based on recombinase polymerase.Frontiers in cellular and infection microbiology · 2024Article
- Do NAAT-Based Methods Increase the Diagnostic Sensitivity ofDiagnostics (Basel, Switzerland) · 2023Article
- Analysis of molecular characteristics of CAMP-negativeFrontiers in microbiology · 2023Article
- Adherence to screening and management guidelines of maternal Group B Streptococcus colonization in pregnancy.Journal of advanced nursing · 2022Article
- An Application of Real-Time PCR and CDC Protocol May Significantly Reduce the Incidence ofPathogens (Basel, Switzerland) · 2022Article
- Assessing standards for prevention of early onset group B streptococcal (GBS) disease in Ireland.Irish journal of medical science · 2022Article
- Use of Recombinant Endolysin to Improve Accuracy of Group B Streptococcus Tests.Microbiology spectrum · 2021Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundStreptococcus Group B (GBS) colonization in pregnant women is the most important risk factor for newborn disease due to vertical transmission during delivery. GBS colonization during pregnancy has been implicated as a leading cause of perinatal infections. Traditionally, pregnant women are screened for GBS between 35 and 37 weeks of gestation. However, antenatal culture-based screening yields no information on GBS colonization status and offers low predictive value for GBS colonization at delivery. Numerous assays have been evaluated for GBS screening in an attempt to validate a fast and efficient method. The aim of this study was to compare bacteria isolation by culture and two qPCR techniques, targeting sip and cfb genes, respectively, for detecting colonizing GBS.
methodsCultures - the gold-standard technique, a previous qPCR technique targeting the sip gene, and a new proposed qPCR assay targeting the cfb gene were evaluated as diagnostic tools on 320 samples.
resultsConsidering cultures as the gold standard, the evaluated qPCR method detected 75 out of 78 samples, representing a sensitivity of 93.58% (95% confidence interval (CI), 90.89-96.27) and specificity of 94.62% (95% CI, 91.78-97.46). However, an additional analysis was performed for true positives that included not only samples showing positives by culture but samples showing positive for both qPCR assays. The sensitivity and specificity were recalculated including these discrepant samples and a total of 89 samples were considered as positive, giving a prevalence of 27.81%. With this new analysis, the qPCR targeting the cfb gene showed a sensitivity of 95.5% (95% CI, 88.65-98.59) and specificity of 99.13% (95% CI, 96.69-99.97).
conclusionsThe new qPCR method is a sensitive and specific assay for detecting GBS colonization and represents a valuable tool for identifying candidates for intrapartum antibiotic prophylaxis. Cultures should be retained as the reference and the routine technique because of its specificity and cost analysis ratio, but it would be convenient to introduce PCR techniques to check negative culture samples or when an urgent detection is required to reduce risk of infection among infants.
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