ArticleDigestive diseases and sciences2018
Effect of Homocysteine on the Differentiation of CD4
Article in Digestive diseases and sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Evaluation of serum and dietary profiles of vitamin B12 and folate and their association with systemic complications in patients with Crohn's disease.European journal of clinical nutrition · 2026Article
- Vitamins as modulators of neonatal intestinal injury.Frontiers in nutrition · 2026Review
- Metabolic modeling reveals a multi-level deregulation of host-microbiome metabolic networks in IBD.Nature communications · 2025Article
- Role of DZ2002 in reducing corneal graft rejection in rats by influencing Th17 activation via inhibition of the PI3K/AKT pathway and downregulation of TRAF1.Open life sciences · 2025Article
- Homocysteine aggravates intestinal inflammation through promotion of 5-LOX and COX-2 in IBD.European journal of medical research · 2024Article
- Homocysteine Promotes Intestinal Inflammation in Colitis Mice Through the PGE2/STAT3 Signaling Pathway.Digestive diseases and sciences · 2024Article
- Effects of Aerobic Treadmill Training on Oxidative Stress Parameters, Metabolic Enzymes, and Histomorphometric Changes in Colon of Rats with Experimentally Induced Hyperhomocysteinemia.International journal of molecular sciences · 2024Article
- Impact of Folate Intake on Bone Mineral Density in Patients with Inflammatory Bowel Disease.Nutrients · 2023Article
- Differential Roles of Cystathionine Gamma-Lyase and Mercaptopyruvate Sulfurtransferase in Hapten-Induced Colitis and Contact Dermatitis in Mice.International journal of molecular sciences · 2023Article
- Review
- Preliminary analysis of immunoregulatory mechanism of hyperhomocysteinemia-induced brain injury in Wistar-Kyoto rats.Experimental and therapeutic medicine · 2021Article
- Hyperhomocysteinemia and Endothelial Dysfunction in Multiple Sclerosis.Brain sciences · 2020Article
- Homocysteine and psoriasis.Bioscience reports · 2019Review
- Micronutrient Absorption and Related Outcomes in People with Inflammatory Bowel Disease: A Review.Nutrients · 2019Review
- Homocysteine and Folate in Inflammatory Bowel Disease: Can Reducing Sulfur Reduce Suffering?Digestive diseases and sciences · 2018Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe hyperhomocysteinaemia (Hhcy) is a common phenomenon observed in patients with inflammatory bowel disease (IBD). Our previous study showed that Hhcy aggravated intestinal inflammation in an animal model of colitis. Increased levels of IL-17 and RORγt were also observed in this animal model of colitis with Hhcy. However, the direct effect of homocysteine on the differentiation of Th17 cells has never been studied. The aim of this study was to investigate the direct effect of Hhcy on the differentiation of CD4
methodLamina propria lymphocytes (LPLs) in colonic mucosa of Wistar rats were isolated and cultured under Th17-inducing (iTH17) environments. Different concentrations of the Hcy (0-100 μmol/ml) were added alone or combined with IL-23 (100 ng/ml) or folate (5 μmol/ml). The LPLs were divided into eight groups as follows: (1) Control group; (2) 10 μmol/ml Hcy group; (3) 25 μmol/ml Hcy group; (4) 50 μmol/ml Hcy group; (5) 100 μmol/ml Hcy group; (6) 100 ng/ml IL-23 group; (7) 50 μmol/ml Hcy + 100 ng/ml IL-23 group and (8) 50 μmol/ml Hcy + 100 ng/ml IL-23 + 5 μmol/ml folate group. The protein expression levels of IL-17, retinoid-related orphan nuclear receptor-γt (RORγt), p38 MAPK, phosphorylated p38 MAPK, cytosolic phospholipase A2 (cPLA2), phosphorylated-cPLA2 and cyclooxygenase 2 (COX2) were detected by immunoblot analysis. The protein level of prostaglandin E2 (PGE2) and IL-17 was detected by ELISA, and IL-17 and RORγt-positive CD4
resultsHcy increased the protein levels of IL-17, RORγt, the ratio of phosphorylated p38 MAPK to p38 MAPK (p-p38/p38), the ratio of phosphorylated cPLA2 to cPLA2 (p-cPLA2/cPLA2) and COX2. The effect was concentration dependent to a certain degree; Hcy of 50 μmol/ml was the optimal concentration to increase the protein levels of those molecules. The level of IL-17 and PGE2 in the cell culture supernatants and the expression of IL-17 and RORγt in positive CD4
conclusionsHomocysteine promoted the differentiation of CD4
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