ArticleCell and tissue research2018
A RNAscope whole mount approach that can be combined with immunofluorescence to quantify differential distribution of mRNA.
Article in Cell and tissue research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 46 citations in OpenAlex.
- Evaluation of the Suitability of RNAscope as a Technique to Measure Gene Expression in Clinical Diagnostics: A Systematic Review.Molecular diagnosis & therapy · 2022Pooled it
- Blood flow patterns in mice are regulated by interpericyte tunneling nanotubes connecting functionally-opposite neuronal areas.Nature communications · 2026Article
- Regulatory Networks Driving the Specification, Differentiation, and Diversification of Neurons in the Mouse Inner Ear.Journal of the Association for Research in Otolaryngology : JARO · 2026Review
- Single-Cell Profiling of the Developing Organ of Corti Identifies Etv4/5/1 as Key Regulators of Pillar Cell Identity.bioRxiv : the preprint server for biology · 2026Article
- Shaping 3D minimal model tissues with mechanical constraints to orchestrate muscle differentiation.Communications biology · 2025Article
- Activation of TRPA1 and TRPM3 triggers CaThe Journal of physiology · 2025Article
- A spatiotemporal cell atlas of cardiopulmonary progenitor cell allocation during development.Cell reports · 2025Article
- Optimizing Factors in Murine Whole-Organ Cochlea Culture.International journal of molecular sciences · 2025Article
- Srebf2 mediates successful optic nerve axon regeneration via the mevalonate synthesis pathway.Molecular neurodegeneration · 2025Article
- RNA-Fluorescence In Situ Hybridization for the Detection of RNA in Mammalian Oocytes and Embryos.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Ucp4 Knockdown of Cerebellar Purkinje Cells Induces Bradykinesia.Molecular neurobiology · 2024Article
- Time space and single-cell resolved tissue lineage trajectories and laterality of body plan at gastrulation.Nature communications · 2023Article
- Article
- Single-molecule RNA in situ detection in clinical FFPE tissue sections by vsmCISH.RNA (New York, N.Y.) · 2023Article
- Gabrb3 is required for the functional integration of pyramidal neuron subtypes in the somatosensory cortex.Neuron · 2023Article
- Efficient RNA and RNA-protein co-detection in 3D colonoids by whole-mount staining.STAR protocols · 2022Article
- BRAFCancers · 2022Article
- Article
- Spatially Resolved and Highly Multiplexed Protein and RNA In Situ Detection by Combining CODEX With RNAscope In Situ Hybridization.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2022Article
- Protocol for RNA fluorescenceSTAR protocols · 2022Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
RNAscope® technology provided by Advanced Cell Diagnostics (ACD) allows the detection and evaluation of coinciding mRNA expression profiles in the same or adjacent cells in unprecedented quantitative detail using multicolor fluorescent in situ hybridization (FISH). While already extensively used in thinly sectioned material of various pathological tissues and, to a lesser extent, in some whole mounts, we provide here a detailed approach to use the fluorescent RNAscope method in the mouse inner ear and thick brain sections by modifying and adapting existing techniques of whole mount fluorescent in situ hybridization (WH-FISH). We show that RNAscope WH-FISH can be used to quantify local variation in overlaying mRNA expression intensity, such as neurotrophin receptors along the length of the mouse cochlea. We also show how RNAscope WH-FISH can be combined with immunofluorescence (IF) of some epitopes that remain after proteinase digestion and, to some extent, with fluorescent protein markers such as tdTomato. Our WH-FISH technique provides an approach to detect cell-specific quantitative differences in developing and mature adjacent cells, an emerging issue revealed by improved cellular expression profiling. Further, the presented technique may be useful in validating single-cell RNAseq data on expression profiles in a range of tissue known or suspected to have locally variable mRNA expression levels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.