ArticleFrontiers in immunology2018
Synthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells.
Article in Frontiers in immunology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Challenges and future directions of AIRR-seq-based diagnostics.Immunoinformatics (Amsterdam, Netherlands) · 2025Article
- Article
- Advancements in mammalian display technology for therapeutic antibody development and beyond: current landscape, challenges, and future prospects.Frontiers in immunology · 2024Review
- TCRpower: quantifying the detection power of T-cell receptor sequencing with a novel computational pipeline calibrated by spike-in sequences.Briefings in bioinformatics · 2022Article
- AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.Methods in molecular biology (Clifton, N.J.) · 2022Article
- TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum.PLoS neglected tropical diseases · 2021Article
- Article
- Biological controls for standardization and interpretation of adaptive immune receptor repertoire profiling.eLife · 2021Review
- Systematic memory B cell archiving and random display shape the human splenic marginal zone throughout life.The Journal of experimental medicine · 2021Article
- A single donor is sufficient to produce a highly functional in vitro antibody library.Communications biology · 2021Article
- Benchmarking of T cell receptor repertoire profiling methods reveals large systematic biases.Nature biotechnology · 2021Article
- Dual UMIs and Dual Barcodes With Minimal PCR Amplification Removes Artifacts and Acquires Accurate Antibody Repertoire.Frontiers in immunology · 2021Article
- Review
- Inter- and intraspecies comparison of phylogenetic fingerprints and sequence diversity of immunoglobulin variable genes.Immunogenetics · 2020Article
- Network Organization of Antibody Interactions in Sequence and Structure Space: the RADARS Model.Antibodies (Basel, Switzerland) · 2020Article
- Automated analysis of immunosequencing datasets reveals novel immunoglobulin D genes across diverse species.PLoS computational biology · 2020Article
- Boosting subdominant neutralizing antibody responses with a computationally designed epitope-focused immunogen.PLoS biology · 2019Article
- Inferred Allelic Variants of Immunoglobulin Receptor Genes: A System for Their Evaluation, Documentation, and Naming.Frontiers in immunology · 2019Review
- Filtering Next-Generation Sequencing of the Ig Gene Repertoire Data Using Antibody Structural Information.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Computational Evaluation of B-Cell Clone Sizes in Bulk Populations.Frontiers in immunology · 2018Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
High-throughput sequencing of immunoglobulin (Ig) repertoires (Ig-seq) is a powerful method for quantitatively interrogating B cell receptor sequence diversity. When applied to human repertoires, Ig-seq provides insight into fundamental immunological questions, and can be implemented in diagnostic and drug discovery projects. However, a major challenge in Ig-seq is ensuring accuracy, as library preparation protocols and sequencing platforms can introduce substantial errors and bias that compromise immunological interpretation. Here, we have established an approach for performing highly accurate human Ig-seq by combining synthetic standards with a comprehensive error and bias correction pipeline. First, we designed a set of 85 synthetic antibody heavy-chain standards (
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