ArticleEpigenetics & chromatin2018
HAM-TBS: high-accuracy methylation measurements via targeted bisulfite sequencing.
Article in Epigenetics & chromatin, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 51 citations in OpenAlex.
- Prenatal exposures and cell type proportions are main drivers ofNeurobiology of stress · 2024Article
- DNA methylation patterns of FKBP5 regulatory regions in brain and blood of humanized mice and humans.Molecular psychiatry · 2024Article
- Methylation Patterns of the FKBP5 Gene in Association with Childhood Maltreatment and Depressive Disorders.International journal of molecular sciences · 2024Article
- FKBP5 intron 7 methylation is associated with higher anxiety proneness and smaller right thalamus volume in adolescents.Brain structure & function · 2022Article
- Betamethasone administration during pregnancy is associated with placental epigenetic changes with implications for inflammation.Clinical epigenetics · 2021Article
- Method of targeted bisulfite massive parallel sequencing of the human LINE-1 retrotransposon promoter.MethodsX · 2021Article
- Investigation of MORC1 DNA methylation as biomarker of early life stress and depressive symptoms.Journal of psychiatric research · 2020Article
- Epigenetic upregulation of FKBP5 by aging and stress contributes to NF-κB-driven inflammation and cardiovascular risk.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Identification of dynamic glucocorticoid-induced methylation changes at the FKBP5 locus.Clinical epigenetics · 2019Article
- Epigenetic Modifications in Stress Response Genes Associated With Childhood Trauma.Frontiers in psychiatry · 2019Review
- Combined neurodevelopmental exposure to deltamethrin and corticosterone is associated with Nr3c1 hypermethylation in the midbrain of male mice.Neurotoxicology and teratologyArticle
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundThe ability to accurately and efficiently measure DNA methylation is critical to advance the understanding of this epigenetic mechanism and its contribution to common diseases. Here, we present a highly accurate method to measure methylation using bisulfite sequencing (termed HAM-TBS). This novel method is able to assess DNA methylation in multiple samples with high accuracy in a cost-effective manner. We developed this assay for the FKBP5 locus, an important gene in the regulation of the stress system and previously linked to stress-related disorders, but the method is applicable to any locus of interest.
resultsHAM-TBS enables multiplexed analyses of up to 96 samples and regions spanning 10 kb using the Illumina MiSeq. It incorporates a triplicate bisulfite conversion step, pooled target enrichment via PCR, PCR-free library preparation and a minimum coverage of 1000×. TBS was able to resolve DNA methylation levels with a mean accuracy of 0.72%. Using this method, we designed and validated a targeted panel to specifically assess regulatory regions within the FKBP5 locus that are not covered in commercially available DNA methylation arrays.
conclusionsHAM-TBS represents a highly accurate, medium-throughput sequencing approach for robust detection of DNA methylation changes in specific target regions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.