Evidence map›Paper›PMID 29973253›Full record

ArticleBMC research notes2018

Detection of Clostridium botulinum group III in environmental samples from farms by real-time PCR using four commercial DNA extraction kits.

Caroline Le Maréchal, Sarah Fourour, Valentine Ballan, Sandra Rouxel, Rozenn Souillard, Marianne Chemaly

Open access · goldAbstract read
In one paragraph

Article in BMC research notes, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.8field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 13 citations in OpenAlex.

  1. Article
  2. Article
  3. Rapid Detection ofInternational journal of environmental research and public health · 2021
    Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Caroline Le MaréchalANSES, Laboratoire de Ploufragan-Plouzané, Unité Hygiène et Qualité des Produits Avicoles et Porcins, Université Bretagne-Loire, BP 53, 22440, Ploufragan, France. caroline.lemarechal@anses.fr.
Sarah FourourANSES, Laboratoire de Ploufragan-Plouzané, Unité Hygiène et Qualité des Produits Avicoles et Porcins, Université Bretagne-Loire, BP 53, 22440, Ploufragan, France.
Valentine BallanANSES, Laboratoire de Ploufragan-Plouzané, Unité Hygiène et Qualité des Produits Avicoles et Porcins, Université Bretagne-Loire, BP 53, 22440, Ploufragan, France.
Sandra RouxelANSES, Laboratoire de Ploufragan-Plouzané, Unité Hygiène et Qualité des Produits Avicoles et Porcins, Université Bretagne-Loire, BP 53, 22440, Ploufragan, France.
Rozenn SouillardANSES, Laboratoire de Ploufragan-Plouzané, Unité d'Épidémiologie et Bien-être en Aviculture et Cuniculture, Université Bretagne-Loire, BP 53, 22440, Ploufragan, France.
Marianne ChemalyANSES, Laboratoire de Ploufragan-Plouzané, Unité Hygiène et Qualité des Produits Avicoles et Porcins, Université Bretagne-Loire, BP 53, 22440, Ploufragan, France.
Université Bretagne Loire · FR

Funding

France AgriMer BOTUNET
6 · The paper itself

Abstract

objectivesFew studies have tested DNA extraction methods to optimize the detection of Clostridium botulinum in environmental samples that can be collected during animal botulism outbreaks. In this study, we evaluated four commercial DNA extraction kits for the detection of C. botulinum group III in 82 various environmental samples (9 manure, 53 swabs, 3 insects, 8 water, 1 silage and 8 soil samples) collected in a context of animal botulism outbreaks.

resultsThe PowerSoil

Indexed as

Real-Time Polymerase Chain ReactionAnimalsClostridium botulinumDNA, BacterialEnvironmental MonitoringFarmsFecesDNA, BacterialClostridium botulinumDetectionDNA extractionFreezingReal-time PCR

Identifiers

PMID29973253
PMCPMC6030735
OpenAlexW2811438569

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.