ArticleBMC microbiology2018
Characterizing interactions of Leptospira interrogans with proximal renal tubule epithelial cells.
Article in BMC microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 51 citations in OpenAlex.
- Proteasome-dependent cytoskeleton disruption during Leptospira interrogans infection induces aberrant collective cell migration.PLoS pathogens · 2026Article
- Protocols for Standard and Advanced Visualization and Quantification of Leptospira Biofilms.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Detection of P1 and P2 Subclades of Leptospira carriage in swine in Sri Lanka.BMC veterinary research · 2025Article
- Distinct strategies of epithelial cell barrier disruption by Leptospira interrogans isolated from human patients in Okinawa, Japan.PLoS neglected tropical diseases · 2025Article
- Review
- Autoimmune Diseases Following Environmental Disasters: A Narrative Review of the Literature.Healthcare (Basel, Switzerland) · 2024Review
- Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin.Veterinary medicine and science · 2024Article
- Impaired functions of human monocyte-derived dendritic cells and induction of regulatory T cells by pathogenic Leptospira.PLoS neglected tropical diseases · 2023Article
- Proteomic profile of naturally released extracellular vesicles secreted from Leptospira interrogans serovar Pomona in response to temperature and osmotic stresses.Scientific reports · 2023Article
- Design, green synthesis, and quorum sensing quenching potential of novel 2-oxo-pyridines containing a thiophene/furan scaffold and targeting aRSC advances · 2023Article
- Epidemiological Characteristics and Trends in the Incidence of Leptospirosis in Japan: A Nationwide, Observational Study from 2006 to 2021.The American journal of tropical medicine and hygiene · 2023Observational
- Case Report: Leptospirosis after a Typhoon Disaster Outside the Endemic Region, Japan.The American journal of tropical medicine and hygiene · 2023Article
- Degradation of p0071 and p120-catenin during adherens junction disassembly byFrontiers in cellular and infection microbiology · 2023Article
- Bovine leptospirosis: effects on reproduction and an approach to research in Colombia.Tropical animal health and production · 2022Review
- Analysis of human clinical and environmental Leptospira to elucidate the eco-epidemiology of leptospirosis in Yaeyama, subtropical Japan.PLoS neglected tropical diseases · 2022Article
- Infectious Uveitis in Horses and New Insights in Its Leptospiral Biofilm-Related Pathogenesis.Microorganisms · 2022Review
- The diagnosis of leptospirosis complicated by pulmonary tuberculosis complemented by metagenomic next-generation sequencing: A case report.Frontiers in cellular and infection microbiology · 2022Article
- Evaluation by polymerase chain reaction assay of persistent shedding of pathogenic leptospires in the urine of dogs with leptospirosis.Journal of veterinary internal medicine · 2022Article
- Understanding the Renal Fibrotic Process in Leptospirosis.International journal of molecular sciences · 2021Review
- In Vivo Biofilm Formation of PathogenicMicroorganisms · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLeptospira interrogans is a pathogenic, spirochetal bacterium that is responsible for leptospirosis, an emerging worldwide zoonosis. Leptospires colonize the renal proximal tubules and chronically infect the kidney. Live bacteria are excreted into urine, contaminating the environment. While it is well known that leptospires can persist in the kidneys without signs of disease for several months, the interactions of leptospires with the proximal renal epithelial tubule cells that allow the chronic renal colonization have not been elucidated yet. In the present study, we compared the interactions between a virulent, low passage (LP) strain and a cultured-attenuated, high passage (HP) strain with renal proximal tubule epithelial cells (RPTECs) to elucidate the strategies used by Leptospira to colonize the kidney.
resultsKinetics analysis of kidney colonization in a mouse model of chronic infection performed by quantitative real-time PCR and immunofluorescence, showed that the LP strain reached the kidney by 3 days post infection (pi) and attached to the basal membrane side of the renal epithelial cells. At 10 days pi, some leptospires were attached to the luminal side of the tubular epithelia and the number of colonizing leptospires gradually increased. On the other hand, the HP strain was cleared during hematogenous dissemination and did not colonize the kidney. Transmission electron microscopy analysis of LP-infected kidneys at 25 days pi showed aggregated leptospires and membrane vesicles attached to the epithelial brush border. Leptospiral kidney colonization altered the organization of the RPTEC brush border. An in vitro model of infection using TCMK-1 cells, showed that leptospiral infection induced a host stress response, which is delayed in LP-infected cells.
conclusionsAfter hematogenous dissemination, leptospires create protective and replicative niches in the base membrane and luminal sides of the RPTECs. During the long-term colonization, leptospires attached to the RPTEC brush borders and membrane vesicles might be involved in the formation of a biofilm-like structure in vivo. Our results also suggested that the virulent strain is able to manipulate host cell stress responses to promote renal colonization.
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