Evidence map›Paper›PMID 29973080›Full record

ArticleJournal of immunotoxicology2018

Methoxychlor metabolite HPTE alters viability and differentiation of embryonic thymocytes from C57BL/6 mice.

Lucie Leung-Gurung, Priscilla Escalante Cobb, Faraj Mourad, Cristina Zambrano, Zachary Muscato, Victoria Sanchez, Kanya Godde, Christine Broussard

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of immunotoxicology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Lucie Leung-Gurunga School of Community and Global Health , Claremont Graduate University , Claremont , CA , USA.
Priscilla Escalante Cobbb Department of Biology , University of La Verne , La Verne , CA , USA.
Faraj Mouradc Irvine Health, University of California , Orange , CA , USA.
Cristina Zambranod Department of Pharmacology, School of Medicine , University of California San Diego , La Jolla , CA , USA.
Zachary Muscatoe Department of Psychiatry, School of Medicine , Oregon Health and Science University , Portland , OR , USA.
Victoria Sanchezb Department of Biology , University of La Verne , La Verne , CA , USA.
Kanya Goddef Department of Sociology/Anthropology , University of La Verne , La Verne , CA , USA.
Christine Broussardb Department of Biology , University of La Verne , La Verne , CA , USA.
University of La Verne · USClaremont Graduate University · USOregon Health & Science University · USUC Irvine Health · USUniversity of California, San Diego · US

Funding

Prenatal Developmental Immunotoxicity of Diethylstilbestrol and MethoxychlorR15ES017345 · NIEHS · UNIVERSITY OF LA VERNE · PI BROUSSARD, CHRISTINE ANN · 2009 to 2011
$328k
NIEHS NIH HHS R15 ES017345
6 · The paper itself

Abstract

Endocrine-disrupting chemicals (EDC) are widespread in the built and natural environments. Heightened public awareness of their potential danger has led to concern about whether EDC and their metabolites have significant negative biological effects. Studies have shown that EDC like DDT and other organochlorine pesticides, such as methoxychlor (MXC), have adverse effects on immune cells, but no studies have addressed the impact of HPTE, the primary metabolite of MXC. To elucidate the presence and significance of HPTE adverse effects, this study explored the impact of HPTE on a critical window and component of immune system development, embryonic T-cell development. Lesions at this phase of development can lead to lifelong immune dysfunction and increased incidence of immune disease, such as autoimmunity. Embry-onic thymocytes (GD 16-18) from C57BL/6 mice were subjected to an in vitro differentiation culture that mimicked early steps in thymocyte development in the presence of 0.005, 0.05, 0.5, 5, or 50 μM HPTE, or a model endocrine disruptor, DES. The results indicated that compared to the vehicle control, HPTE- and DES-induced death of thymocytes. Annexin-V staining and Caspase 8, markers of programed cell death, revealed that the loss of cells was due at least in part to induction of apoptosis. Moreover, HPTE-induced cell death not only resulted in selective loss of double positive thymocytes, but also loss of developing CD4 intermediate cells (post-double positive partially differentiated thymocyte population). Phenotypic analysis of thymocyte maturation (T-cell receptor, TCR) and TCR ligation (CD5) surface markers revealed that surviving embryonic thymocytes expressed low levels of both. Taken together these data demonstrate that immature embryonic thymocytes are sensitive to HPTE exposure and that HPTE exposure targets thymocyte populations undergoing critical differentiation steps. These findings suggest HPTE may play a pivotal role in MXC exposure-induced immune dysfunction.

Indexed as

AnimalsCell DifferentiationCells, CulturedCell SurvivalEndocrine DisruptorsFemaleFetal DevelopmentFetusHumansMaleMethoxychlorMiceMice, Inbred C57BLPesticidesPhenolsThymocytes2,2-bis(4-hydroxyphenyl)-1,1,1-trichloroethaneEndocrine DisruptorsMethoxychlorPesticidesPhenolsapoptosisCD5embryonic developmentembryonic thymocyteendocrine disruptorHPTEMethoxychlorT-cell developmentthymocyte differentiation

Identifiers

PMID29973080
PMCPMC6120686
OpenAlexW2837760300

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.