ArticleCell reports2018
Classification of Single Particles from Human Cell Extract Reveals Distinct Structures.
Article in Cell reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Confidence-guided cryo-EM map optimisation with LocScale-2.0.Nature communications · 2026Article
- Capturing ribosomal structures in cellular extracts with cryoPRISM: A purification-free cryoEM approach reveals novel structural states.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
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- Serendipity and the Slime Mold: A Visual Survey of High-Molecular-Weight Protein Assemblies Reveals the Structure of the Polyketide Synthase Pks16.Molecular & cellular proteomics : MCP · 2026Article
- MagIC beads for scarce macromolecules.eLife · 2025Article
- Review
- Algebraic constraints and algorithms for common lines in cryo-EM.Biological imaging · 2024Article
- Moment-based metrics for molecules computable from cryogenic electron microscopy images.Biological imaging · 2024Article
- Structural biology of an organ.Cell reports · 2023Article
- The protein organization of a red blood cell.Cell reports · 2022Article
- Characterizing Endogenous Protein Complexes with Biological Mass Spectrometry.Chemical reviews · 2022Review
- Label-free visual proteomics: Coupling MS- and EM-based approaches in structural biology.Molecular cell · 2022Review
- Structural features of nucleosomes in interphase and metaphase chromosomes.Molecular cell · 2021Article
- Cryo-electron microscopy of cholinesterases, present and future.Journal of neurochemistry · 2021Review
- Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy.Nanoscale · 2021Article
- Determination of the Near-Atomic Structure of Non-Purified OligomericCrystallography reports · 2021Article
- Detecting Protein Communities in Native Cell Extracts by Machine Learning: A Structural Biologist's Perspective.Frontiers in molecular biosciences · 2021Article
- Cryo-EM as a tool to study bacterial efflux systems and the membrane proteome.Faculty reviews · 2021Review
- EMPAS: Electron Microscopy Screening for Endogenous Protein Architectures.Molecules and cells · 2020Article
- Structural Biology in the Multi-Omics Era.Journal of chemical information and modeling · 2020Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Multi-protein complexes are necessary for nearly all cellular processes, and understanding their structure is required for elucidating their function. Current high-resolution strategies in structural biology are effective but lag behind other fields (e.g., genomics and proteomics) due to their reliance on purified samples rather than heterogeneous mixtures. Here, we present a method combining single-particle analysis by electron microscopy with protein identification by mass spectrometry to structurally characterize macromolecular complexes from human cell extract. We identify HSP60 through two-dimensional classification and obtain three-dimensional structures of native proteasomes directly from ab initio classification of a heterogeneous mixture of protein complexes. In addition, we reveal an ∼1-MDa-size structure of unknown composition and reference our proteomics data to suggest possible identities. Our study shows the power of using a shotgun approach to electron microscopy (shotgun EM) when coupled with mass spectrometry as a tool to uncover the structures of macromolecular machines.
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Registered trials
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