Evidence map›Paper›PMID 29971716›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2018

High-Resolution Single-Molecule Kinesin Assays at kHz Frame Rates.

Keith J Mickolajczyk, William O Hancock

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Cascaded momentum-space polarization filters enabled label-free black-field microscopy for single nanoparticles analysis.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Keith J MickolajczykBiomedical Engineering Department, Pennsylvania State University, University Park, PA, USA.
William O HancockBiomedical Engineering Department, Pennsylvania State University, University Park, PA, USA. wohbio@engr.psu.edu.

Funding

Molecular mechanism of kinesin motilityR01GM076476 · NIGMS · PENNSYLVANIA STATE UNIVERSITY, THE · PI HANCOCK, WILLIAM OLAF · 2006 to 2019
$3.5M
A biophysical approach to elucidating the molecular mechanisms of mitotic inhibitor targetsF99CA223018 · NCI · PENNSYLVANIA STATE UNIVERSITY, THE · PI MICKOLAJCZYK, KEITH JOSEPH · 2017 to 2017
$33k
NCI NIH HHS F99 CA223018NIGMS NIH HHS R01 GM076476
6 · The paper itself

Abstract

This chapter describes methods for high-speed, unloaded, in vitro single-molecule kinesin tracking experiments. Instructions are presented for constructing a total internal reflection dark-field microscope (TIRDFM) and labeling motors with gold nanoparticles. An AMP-PNP unlocking assay is introduced as a specialized means of capturing processive events in a reduced field of view. Finally, step-finding tools for analyzing high frame-rate tracking data are described.

Indexed as

Adenylyl ImidodiphosphateAnimalsBiological AssayCalibrationCattleData AnalysisDrosophila melanogasterKinesinsMicrotubulesSingle Molecule ImagingAdenylyl ImidodiphosphateKinesinsGold nanoparticleKinesinSingle-moleculeTotal internal reflection dark-field microscopy

Identifiers

PMID29971716
PMCPMC7851763

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.