ArticleInternational journal of molecular sciences2018
Red (635 nm), Near-Infrared (808 nm) and Violet-Blue (405 nm) Photobiomodulation Potentiality on Human Osteoblasts and Mesenchymal Stromal Cells: A Morphological and Molecular In Vitro Study.
Article in International journal of molecular sciences, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers, 6 of them syntheses that pooled it.
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The trial behind it
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Who cites it
51 citing papers in PubMed, 6 syntheses or guidelines pooled it.
- Violet photobiomodulation: biological effects and health implications.Lasers in medical science · 2025Pooled it
- The implication of blue light-emitting diode on mesenchymal stem cells: a systematic review.Lasers in medical science · 2023Pooled it
- The Role of Low-Level Laser Therapy in Bone Healing: Systematic Review.International journal of molecular sciences · 2023Pooled it
- Effects of low-level laser therapy on the orthodontic mini-implants stability: a systematic review and meta-analysis.Progress in orthodontics · 2021Pooled it
- NIR light-assisted phototherapies for bone-related diseases and bone tissue regeneration: A systematic review.Theranostics · 2020Pooled it
- Photonic Therapy in Periodontal Diseases an Overview with Appraisal of the Literature and Reasoned Treatment Recommendations.International journal of molecular sciences · 2019Pooled it
- Photobiomodulation Therapy on Orthodontic Movement: Analysis of Preliminary Studies with a New Protocol.International journal of environmental research and public health · 2020Trial
- Review
- Biphasic energy dependent effects of 650 nm diode laser photobiomodulation on orthodontic tooth movement and compression zone bone remodeling in vivo.BMC oral health · 2026Article
- Heterologous Fibrin Biopolymer as a Key Scaffold for Bone Regeneration: Synergistic Effects with Photobiomodulation and Membrane Therapy.Gels (Basel, Switzerland) · 2026Article
- Red single wavelength emitting up-conversion nanoparticles modulate cellular dynamics and gene expression in T24 bladder cancer cells.Scientific reports · 2025Article
- Shining a Light on Skeletal Muscle Regeneration: Red Photobiomodulation Boosts Myoblast Differentiation In Vitro.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Assessment of photobiomodulation effect on osteoblast-like MG-63 cell biological behavior: analyzing proliferation and migration in the absence or presence of nicotine.BMC oral health · 2025Article
- Photobiomodulation promotes osteogenic differentiation of mesenchymal stem cells and increases P-Akt levels in vitro.Scientific reports · 2025Article
- Evaluation of the Effects of Photobiomodulation on Bone Density After Placing Dental Implants: A Pilot Study Using Cone Beam CT Analysis.Clinics and practice · 2025Article
- Effect of nutritional stress and photobiomodulation protocol on in vitro viability and proliferation of murine preosteoblast cells.Lasers in medical science · 2024Article
- Photobiomodulation with laser and led on mesenchymal stem cells viability and wound closure in vitro.Lasers in medical science · 2024Article
- The effect of continuous long-term illumination with visible light in different spectral ranges on mammalian cells.Scientific reports · 2024Article
- Osteoblastic differentiation and changes in the redox state in pulp stem cells by laser treatment.Lasers in medical science · 2024Article
- Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Photobiomodulation (PBM) has been used for bone regenerative purposes in different fields of medicine and dentistry, but contradictory results demand a skeptical look for its potential benefits. This in vitro study compared PBM potentiality by red (635 ± 5 nm) or near-infrared (NIR, 808 ± 10 nm) diode lasers and violet-blue (405 ± 5 nm) light-emitting diode operating in a continuous wave with a 0.4 J/cm² energy density, on human osteoblast and mesenchymal stromal cell (hMSC) viability, proliferation, adhesion and osteogenic differentiation. PBM treatments did not alter viability (PI/Syto16 and MTS assays). Confocal immunofluorescence and RT-PCR analyses indicated that red PBM (i) on both cell types increased vinculin-rich clusters, osteogenic markers expression (Runx-2, alkaline phosphatase, osteopontin) and mineralized bone-like nodule structure deposition and (ii) on hMSCs induced stress fiber formation and upregulated the expression of proliferation marker Ki67. Interestingly, osteoblast responses to red light were mediated by Akt signaling activation, which seems to positively modulate reactive oxygen species levels. Violet-blue light-irradiated cells behaved essentially as untreated ones and NIR irradiated ones displayed modifications of cytoskeleton assembly, Runx-2 expression and mineralization pattern. Although within the limitations of an in vitro experimentation, this study may suggest PBM with 635 nm laser as potential effective option for promoting/improving bone regeneration.
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