ArticleParasites & vectors2018
Screening for biomarkers reflecting the progression of Babesia microti infection.
Article in Parasites & vectors, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 32 citations in OpenAlex.
- Research progress on diagnostic techniques for differentFrontiers in cellular and infection microbiology · 2025Review
- Transmission risk evaluation of transfusion blood containing low-densityFrontiers in cellular and infection microbiology · 2024Article
- A conserved protein of Babesia microti elicits partial protection against Babesia and Plasmodium infection.Parasites & vectors · 2023Article
- Advances inPathogens (Basel, Switzerland) · 2023Review
- A Fluorescent Recombinase Aided Amplification Assay for Detection of Babesia microti.The Korean journal of parasitology · 2022Article
- Phosphorylation regulation of cardiac proteins in Babesia microti infected mice in an effort to restore heart function.Parasites & vectors · 2022Article
- Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid.The Korean journal of parasitology · 2021Article
- Exploration of Serum Marker Proteins in Mice Induced by Babesia microti Infection Using a Quantitative Proteomic Approach.The protein journal · 2021Article
- Review
- Oncogenic action of the exosome cofactor RBM7 by stabilization of CDK1 mRNA in breast cancer.NPJ breast cancer · 2020Article
- Article
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Authors and funding
19 authors at 5 institutions in 2 countries.
Funding
Abstract
backgroundBabesiosis is caused by the invasion of erythrocytes by parasites of the Babesia spp. Babesia microti is one of the primary causative agents of human babesiosis. To better understand the status of the disease, discovering key biomarkers of the different infection stages is crucial.
resultsThis study investigated B. microti infection in the mouse model from 0 to 270 days post-infection (dpi), using blood smears, PCR assays and ELISA. PCR assays showed a higher sensitivity when compared to microscopic examination. Specific IgG antibodies could be detected from 7 days to 270 dpi. Two-dimensional electrophoresis was combined with western blotting and mass spectrometric analysis to screen for specific reactive antigens during both the peak parasitaemia period (7 dpi) and IgG antibody response peak period (30 dpi) by the infected mice plasma. The 87 positive reactive proteins were identified and then expressed with the wheat germ cell-free system. Protein microarrays of all 87 targeted proteins were produced and hybridized with the serial plasma of infected mice model. Based on the antigen reaction profile during the infection procedure, 6 antigens were selected and expressed in Escherichia coli. Due to an early response to IgM, lower immunoreactivity levels of IgG after two months and higher immunoreactivity level IgG during nine months, four recombinant proteins were selected for further characterization, namely rBm2D97(CCF75281.1), rBm2D33(CCF74637.1), rBm2D41(CCF75408.1) and rBm7(CCF73510.1). The diagnostic efficacy of the four recombinant protein candidates was evaluated in a clinical setting using babesiosis patient plasma. The rBm2D33 showed the highest sensitivity with a positive rate of 62.5%. Additional characterization of the two candidate proteins using a mouse vaccination assay, demonstrated that rBm2D41 could reduce peak parasitaemia by 37.4%, indicating its efficacy in preventing severe babesiosis.
conclusionsThe detection technologies of microscopic examination, PCR assays and antibody tests showed different sensitivities and accuracy during the different stages of B. microti infection. Antibody detection has a unique significance for B. microti infection in the asymptomatic stages. Using immunoreactivity profiles, biomarkers for disease progression were identified and represent useful information for future the diagnosis and vaccine development for this serious disease of public health significance.
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