ArticleJournal of dental research2018
Activity of Streptococcus mutans VicR Is Modulated by Antisense RNA.
Article in Journal of dental research, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 75 citations in OpenAlex.
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- Terahertz Imaging Detects Oral Cariogenic Microbial Domains Characteristics.Journal of dental research · 2024Article
- SepM mutation in Streptococcus mutans clinical isolates and related function analysis.BMC oral health · 2024Article
- Six Spain Thymus essential oils composition analysis and their in vitro and in silico study against Streptococcus mutans.BMC complementary medicine and therapies · 2023Article
- Regulation of biofilm formation by non-coding RNA in prokaryotes.Current research in pharmacology and drug discovery · 2023Review
- Antimicrobial resistance and mechanisms of epigenetic regulation.Frontiers in cellular and infection microbiology · 2023Review
- Regulatory mechanisms of exopolysaccharide synthesis and biofilm formation in Streptococcus mutans.Journal of oral microbiology · 2023Review
- Exopolysaccharides metabolism and cariogenesis ofJournal of oral microbiology · 2023Article
- In Vitro Studies of Graphene for Management of Dental Caries and Periodontal Disease: A Concise Review.Pharmaceutics · 2022Review
- Enterococcus faecalis rnc gene modulates its susceptibility to disinfection agents: a novel approach against biofilm.BMC oral health · 2022Article
- Research on Graphene and Its Derivatives in Oral Disease Treatment.International journal of molecular sciences · 2022Review
- Analysis ofFrontiers in microbiology · 2022Article
- Mini Review Therapeutic Strategies Targeting for Biofilm and Bone Infections.Frontiers in microbiology · 2022Review
- Strategies for dispersion of cariogenic biofilms: applications and mechanisms.Frontiers in microbiology · 2022Review
- RNase III coding genes modulate the cross-kingdom biofilm ofFrontiers in microbiology · 2022Article
- AntisenseInternational journal of nanomedicine · 2022Article
- CodY is modulated by YycF and affects biofilm formation inFrontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
The VicRK 2-component system of Streptococcus mutans regulates genes associated with cell wall biogenesis and biofilm formation. A putative RNase III-encoding gene ( rnc) is located downstream from the vicRKX operon. The goals of this study were to investigate the potential role of VicR in the regulation of adjacent downstream genes and evaluate transcription levels of vicR during planktonic and biofilm growth. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to investigate whether vicRKX and adjacent downstream genes were cotranscribed. Binding of purified recombinant VicR protein to promoter regions of vicR, rnc, and syfA genes was confirmed by electrophoretic mobility shift assay and by chromatin immunoprecipitation analyses. VicR antisense (AS vicR) RNA was detected by Northern blotting and qRT-PCR assays. AS vicR overexpression mutants were constructed, and the biofilm biomass was determined by crystal violet microtiter assay. Adjacent downstream genes rnc, smc, syfA, smu.1511, and syfB were cotranscribed with vicRKX. The predicted promoter regions of vicR, rnc, and syfA genes were directly regulated by VicR. An AS vicR RNA transcript was detected upstream of the rnc gene. Expression of the AS vicR RNA transcript was elevated in planktonic cultures and repressed during biofilm growth. In addition, Western blot data showed that expression of the VicR protein decreased by 35% in planktonic as compared with biofilm cultures. Furthermore, we show that overexpression of AS vicR led to a reduction in biofilm formation. The downstream genes rnc, smc, syfA, smu.1511, and syfB are cotranscribed with vicRKX. VicR is autophosphorylated, and rnc and syfA are directly regulated by VicR. Expression of VicR protein correlated inversely with different levels of AS vicR RNA transcript and growth conditions. The biofilm biomass decreased in the AS vicR overexpression mutant. These data suggest a role for the AS vicR RNA transcript in posttranscriptional regulation of VicR protein production in S. mutans.
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