ArticleAnalytical chemistry2018
Simple and Precise Counting of Viable Bacteria by Resazurin-Amplified Picoarray Detection.
Article in Analytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- A digital droplet microarray for measuring tolerance to antibiotics.bioRxiv : the preprint server for biology · 2026Article
- Droplet-Based Microfluidics in Single-Bacterium Analysis: Advancements in Cultivation, Detection, and Application.Biosensors · 2025Review
- A digital plating platform for robust and versatile microbial detection and analysis.Scientific reports · 2025Article
- Cross-kingdom pathogen detection via duplex universal PCR and high-resolution melt.Biosensors & bioelectronics · 2025Article
- Spectroscopic characterization of bacterial colonies through UV hyperspectral imaging techniques.Frontiers in chemistry · 2025Article
- Urinary Tract Infection Diagnosis Among People With Neurogenic Lower Urinary Tract Infection Due to Spinal Cord Injury and Disease: Time for a Change.Topics in spinal cord injury rehabilitation · 2025Review
- Modeling of Electric Field and Dielectrophoretic Force in a Parallel-Plate Cell Separation Device with an Electrode Lid and Analytical Formulation Using Fourier Series.Sensors (Basel, Switzerland) · 2024Article
- Application of the Electrical Microbial Growth Analyzer Method for Efficiently Quantifying Viable Bacteria in Ready-to-Eat Sea Cucumber Products.Microorganisms · 2024Article
- Automated and miniaturized screening of antibiotic combinationsActa pharmaceutica Sinica. B · 2024Article
- Harnessing Variabilities in Digital Melt Curves for Accurate Identification of Bacteria.Analytical chemistry · 2023Article
- Multiplex Digital Methylation-Specific PCR for Noninvasive Screening of Lung Cancer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Moving microcapillary antibiotic susceptibility testing (mcAST) towards the clinic: unravelling kinetics of detection of uropathogenicSensors & diagnostics · 2023Article
- [Application of Droplet-Based Microfluidics in Microbial Research].Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition · 2023Review
- Improving droplet microfluidic systems for studying single bacteria growth.Analytical and bioanalytical chemistry · 2023Article
- Accelerating the Detection of Bacteria in Food Using Artificial Intelligence and Optical Imaging.Applied and environmental microbiology · 2023Article
- Single-cell pathogen diagnostics for combating antibiotic resistance.Nature reviews. Methods primers · 2023Article
- Emerging platforms for high-throughput enzymatic bioassays.Trends in biotechnology · 2023Review
- Microfluidic One-Pot Digital Droplet FISH Using LNA/DNA Molecular Beacons for Bacteria Detection and Absolute Quantification.Biosensors · 2022Article
- Combinatorial nanodroplet platform for screening antibiotic combinations.Lab on a chip · 2022Article
- Combating Antimicrobial Resistance via Single-Cell Diagnostic Technologies Powered by Droplet Microfluidics.Accounts of chemical research · 2022Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Simple, fast, and precise counting of viable bacteria is fundamental to a variety of microbiological applications such as food quality monitoring and clinical diagnosis. To this end, agar plating, microscopy, and emerging microfluidic devices for single bacteria detection have provided useful means for counting viable bacteria, but they also have their limitations ranging from complexity, time, and inaccuracy. We present herein our new method RAPiD (Resazurin-Amplified Picoarray Detection) for addressing this important problem. In RAPiD, we employ vacuum-assisted sample loading and oil-driven sample digitization to stochastically confine single bacteria in Picoarray, a microfluidic device with picoliter-sized isolation chambers (picochambers), in <30 s with only a few minutes of hands-on time. We add AlamarBlue, a resazurin-based fluorescent dye for bacterial growth, in our assay to accelerate the detection of "microcolonies" proliferated from single bacteria within picochambers. Detecting fluorescence in picochambers as an amplified surrogate for bacterial cells allows us to count hundreds of microcolonies with a single image taken via wide-field fluorescence microscopy. We have also expanded our method to practically test multiple titrations from a single bacterial sample in parallel. Using this expanded "multi-RAPiD" strategy, we can quantify viable cells in E. coli and S. aureus samples with precision in ∼3 h, illustrating RAPiD as a promising new method for counting viable bacteria for microbiological applications.
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