ArticleACS synthetic biology2018
Mutually Orthogonal DNA Replication Systems In Vivo.
Article in ACS synthetic biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Exploring the Evolutionary Landscape with Targeted In Vivo Hypermutations.Biomedicines · 2026Review
- Evidence that Zymocin-Like Killer Plasmids Were Present in the Common Ancestor of Terrestrial Fungi.Genome biology and evolution · 2026Article
- Ultra-efficient Integration of Gene Libraries onto Yeast Cytosolic Plasmids.ACS synthetic biology · 2025Article
- High throughput mutagenesis and screening for yeast engineering.Journal of biological engineering · 2022Review
- Hypermutation of specific genomic loci of Pseudomonas putida for continuous evolution of target genes.Microbial biotechnology · 2022Article
- In vivo hypermutation and continuous evolution.Nature reviews. Methods primers · 2022Article
- Reprogramming the genetic code.Nature reviews. Genetics · 2021Review
- Optimizing Calcium Detection Methods in Animal Systems: A Sandbox for Synthetic Biology.Biomolecules · 2021Review
- Winner-takes-all resource competition redirects cascading cell fate transitions.Nature communications · 2021Article
- Synthetic Biological Circuits within an Orthogonal Central Dogma.Trends in biotechnology · 2021Review
- Topology-dependent interference of synthetic gene circuit function by growth feedback.Nature chemical biology · 2020Article
- Engineering Polymerases for New Functions.Trends in biotechnology · 2019Review
- Genetic Compatibility and Extensibility of Orthogonal Replication.ACS synthetic biology · 2019Article
- In vivo continuous evolution of metabolic pathways for chemical production.Microbial cell factories · 2019Review
- Synthetic biology for evolutionary engineering: from perturbation of genotype to acquisition of desired phenotype.Biotechnology for biofuels · 2019Review
- An Adaptable Platform for Directed Evolution in Human Cells.Journal of the American Chemical Society · 2018Article
- Tunable Expression Systems for Orthogonal DNA Replication.ACS synthetic biology · 2018Article
- Scalable, Continuous Evolution of Genes at Mutation Rates above Genomic Error Thresholds.Cell · 2018Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The yeast cytoplasmically localized pGKL1/TP-DNAP1 plasmid/DNA polymerase pair forms an orthogonal DNA replication system whose mutation rate can be drastically increased without influencing genomic replication, thereby supporting in vivo continuous evolution. Here, we report that the pGKL2/TP-DNAP2 plasmid/DNA polymerase pair forms a second orthogonal replication system. We show that custom genes can be encoded and expressed from pGKL2, that error-prone TP-DNAP2s can be engineered, and that pGKL2 replication by TP-DNAP2 is both orthogonal to genomic replication in Saccharomyces cerevisiae and mutually orthogonal with pGKL1 replication by TP-DNAP1. This demonstration of two mutually orthogonal DNA replication systems with tunable error rates and properties should enable new applications in cell-based continuous evolution, genetic recording, and synthetic biology at large.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.