ArticleScientific reports2018
SSPIM: a beam shaping toolbox for structured selective plane illumination microscopy.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology.Nature methods · 2025Article
- Multicolor iLIFE (m-iLIFE) volume cytometry for high-throughput imaging of multiple organelles.Scientific reports · 2024Article
- Bessel light beam for a surgical laser focusing telescope-a novel approach.Lasers in medical science · 2024Article
- Light sheet fluorescence microscopy for the investigation of blood-sucking arthropods dyed via artificial membrane feeding.Parasites & vectors · 2022Article
- Bessel Beam: Significance and Applications-A Progressive Review.Micromachines · 2020Review
- Multi-purpose SLM-light-sheet microscope.Biomedical optics express · 2018Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Selective plane illumination microscopy (SPIM) represents a preferred method in dynamic tissue imaging, because it combines high spatiotemporal resolution with low phototoxicity. The OpenSPIM system was developed to provide an accessible and flexible microscope set-up for non-specialist users. Here, we report Structured SPIM (SSPIM), which offers an open-source, user-friendly and compact toolbox for beam shaping to be applied within the OpenSPIM platform. SSPIM is able to generate digital patterns for a wide range of illumination beams including static and spherical Gaussian beams, Bessel beams and Airy beams by controlling the pattern of a Spatial Light Modulator (SLM). In addition, SSPIM can produce patterns for structured illumination including incoherent and coherent array beams and tiling for all types of the supported beams. We describe the workflow of the toolbox and demonstrate its application by comparing experimental data with simulation results for a wide range of illumination beams. Finally, the capability of SSPIM is investigated by 3D imaging of Drosophila embryos using scanned Gaussian, Bessel and array beams. SSPIM provides an accessible toolbox to generate and optimize the desired beam patterns and helps adapting the OpenSPIM system towards a wider range of biological samples.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.