ArticleScientific reports2018
Entomological signatures in honey: an environmental DNA metabarcoding approach can disclose information on plant-sucking insects in agricultural and forest landscapes.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Pooled it
- Honey environmental DNA reveals entomological fingerprints through dual mitochondrial cytochrome c oxidase subunit 1 (COI) and cytochrome b (CYTB) metabarcoding.Scientific reports · 2026Article
- Biogeography from a food matrix: a temporal distribution map of Apis mellifera mitochondrial DNA lineages across Italy, obtained from honey samples.Scientific reports · 2026Article
- A Tandem Metabarcoding and Taxonomic Forensics Approach to Surveillance of Mosquito-Plant Interactions forInsects · 2025Article
- Sapsucker Wells as a Keystone Nutritional Resource: Evaluating Methods for Detection of Secondary Sap Consumers.Ecology and evolution · 2025Article
- Development of an LNA-Based qPCR Assay for DetectingInsects · 2025Article
- Italian Honeydew Honey Characterization byFoods (Basel, Switzerland) · 2025Article
- Honey bulk DNA metagenomic analysis to identify honey biological composition and monitor honey bee pathogens.NPJ science of food · 2025Article
- The buzz about honey-based biosurveys.npj biodiversity · 2024Review
- A preliminary metabarcoding analysis of Portuguese raw honeys.Archives of microbiology · 2023Article
- Non-destructive collection and metabarcoding of arthropod environmental DNA remained on a terrestrial plant.Scientific reports · 2023Article
- Towards DNA-Based Methods Analysis for Honey: An Update.Molecules (Basel, Switzerland) · 2023Review
- A genotyping by sequencing approach can disclose Apis mellifera population genomic information contained in honey environmental DNA.Scientific reports · 2022Article
- Biochemical Reactions and Their Biological Contributions in Honey.Molecules (Basel, Switzerland) · 2022Review
- Honey Environmental DNA Can Be Used to Detect and Monitor Honey Bee Pests: Development of Methods Useful to IdentifyVeterinary sciences · 2022Article
- The Honey BeeBiology · 2022Review
- Geographical Distribution and Long-Term Monitoring ofInsects · 2021Article
- Application of Next Generation Semiconductor-Based Sequencing for the Identification ofInsects · 2021Article
- Article
- Shotgun sequencing of honey DNA can describe honey bee derived environmental signatures and the honey bee hologenome complexity.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Honeydew produced from the excretion of plant-sucking insects (order Hemiptera) is a carbohydrate-rich material that is foraged by honey bees to integrate their diets. In this study, we used DNA extracted from honey as a source of environmental DNA to disclose its entomological signature determined by honeydew producing Hemiptera that was recovered not only from honeydew honey but also from blossom honey. We designed PCR primers that amplified a fragment of mitochondrial cytochrome c oxidase subunit 1 (COI) gene of Hemiptera species using DNA isolated from unifloral, polyfloral and honeydew honeys. Ion Torrent next generation sequencing metabarcoding data analysis assigned Hemiptera species using a customized bioinformatic pipeline. The forest honeydew honeys reported the presence of high abundance of Cinara pectinatae DNA, confirming their silver fir forest origin. In all other honeys, most of the sequenced reads were from the planthopper Metcalfa pruinosa for which it was possible to evaluate the frequency of different mitotypes. Aphids of other species were identified from honeys of different geographical and botanical origins. This unique entomological signature derived by environmental DNA contained in honey opens new applications for honey authentication and to disclose and monitor the ecology of plant-sucking insects in agricultural and forest landscapes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.