Evidence map›Paper›PMID 29968174›Full record

ArticleJournal of mammary gland biology and neoplasia2018

Polarized Secretion of Extracellular Vesicles by Mammary Epithelia.

Andrew R Chin, Wei Yan, Minghui Cao, Xuxiang Liu, Shizhen Emily Wang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of mammary gland biology and neoplasia, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
  2. Review
  3. Microbiology spectrum · 2024
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  6. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Andrew R ChinDepartment of Pathology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0612, USA.
Wei YanDepartment of Pathology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0612, USA.
Minghui CaoDepartment of Pathology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0612, USA.
Xuxiang LiuCity of Hope Irell & Manella Graduate School of Biological Sciences, Duarte, CA, 91010, USA.
Shizhen Emily WangDepartment of Pathology, University of California San Diego, 9500 Gilman Drive, La Jolla, CA, 92093-0612, USA. emilywang@ucsd.edu.ORCID 0000-0002-5036-8175
University of California San Diego · USCity of Hope · US

Funding

VIRAL MALIGNANCYP30CA023100 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI DIANE M SIMEONE · 1985 to 2026
$124.9M
Transgenic Mouse FacilityP30CA033572 · NCI · CITY OF HOPE/BECKMAN RESEARCH INSTITUTE · PI STANLEY R. HAMILTON · 1985 to 2026
$86.3M
Role of breast cancer-secreted miRNA in directing a stromal metabolic plasticityR01CA218140 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI WANG, SHIZHEN EMILY · 2017 to 2021
$2.4M
Targeting Chemotherapy-induced Breast Cancer StemnessR01CA206911 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI WANG, SHIZHEN EMILY, YUAN, YUAN · 2017 to 2021
$2.3M
Role of miR-105 in breast cancer metastasisR01CA166020 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI WANG, SHIZHEN EMILY · 2012 to 2016
$1.7M
NCI NIH HHS P30 CA023100NCI NIH HHS P30 CA033572NCI NIH HHS R01 CA166020NCI NIH HHS R01 CA206911NCI NIH HHS R01 CA218140
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are secreted by many cell types and are increasingly investigated for their role in human diseases including cancer. Here we focus on the secretion and potential physiological function of non-pathological EVs secreted by polarized normal mammary epithelial cells. Using a transwell system to allow formation of epithelial polarity and EV collection from the apical versus basolateral compartments, we found that impaired secretion of EVs by knockdown of RAB27A or RAB27B suppressed the establishment of mammary epithelial polarity, and that addition of apical but not basolateral EVs suppressed epithelial polarity in a dose-dependent manner. This suggests that apical EV secretion contributes to epithelial polarity, and a possible mechanism is through removal of certain intracellular molecules. In contrast, basolateral but not apical EVs promoted migration of mammary epithelial cells in a motility assay. The protein contents of apical and basolateral EVs from MCF10A and primary human mammary epithelial cells were determined by mass spectrometry proteomic analysis, identifying apical-EV-enriched and basolateral-EV-enriched proteins that may contribute to different physiological functions. Most of these proteins differentially secreted by normal mammary epithelial cells through polarized EV release no longer showed polarized secretion in MCF10A-derived transformed epithelial cells. Our results suggest an essential role of EV secretion in normal mammary epithelial polarization and distinct protein contents and functions in apical versus basolateral EVs secreted by polarized mammary epithelia.

Indexed as

Cell LineCell Line, TumorCell MovementCell PolarityEpithelial CellsEpitheliumExtracellular VesiclesFemaleHumansMammary Glands, HumanProteomicsrab GTP-Binding Proteinsrab GTP-Binding ProteinsEpithelial polarizationExtracellular vesiclesMammary glandProteomics

Identifiers

PMID29968174
PMCPMC6103817
OpenAlexW2810270993

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.