ArticleAnalytical and bioanalytical chemistry2018
Graphene oxide-based biosensing platform for rapid and sensitive detection of HIV-1 protease.
Article in Analytical and bioanalytical chemistry, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 49 citations in OpenAlex.
- The Application of Hybridization Chain Reaction in the Detection of Foodborne Pathogens.Foods (Basel, Switzerland) · 2023Review
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- Proteolytic Biosensors with Functional Nanomaterials: Current Approaches and Future Challenges.Biosensors · 2023Review
- Genosensors as an alternative diagnostic sensing approaches for specific detection of virus species: A review of common techniques and outcomes.Trends in analytical chemistry : TRAC · 2022Review
- Fluorescent Biosensors for the Detection of Viruses Using Graphene and Two-Dimensional Carbon Nanomaterials.Biosensors · 2022Review
- Article
- Joint Entropy-Assisted Graphene Oxide-Based Multiplexing Biosensing Platform for Simultaneous Detection of Multiple Proteases.Analytical chemistry · 2020Article
- Graphene biosensors for bacterial and viral pathogens.Biosensors & bioelectronics · 2020Review
- Chemically functionalized conical PET nanopore for protein detection at the single-molecule level.Biosensors & bioelectronics · 2020Article
- Robust Magnetized Graphene Oxide Platform for In Situ Peptide Synthesis and FRET-Based Protease Detection.Sensors (Basel, Switzerland) · 2020Article
- A FRET assay for the quantitation of inhibitors of exonuclease EcoRV by using parchment paper inkjet-printed with graphene oxide and FAM-labelled DNA.Mikrochimica acta · 2019Article
- Rapid and sensitive detection of the activity of ADAM17 using a graphene oxide-based fluorescence sensor.The Analyst · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
HIV-1 protease is essential for the life cycle of the human immunodeficiency virus (HIV), and is one of the most important clinical targets for antiretroviral therapies. In this work, we developed a graphene oxide (GO)-based fluorescence biosensing platform for the rapid, sensitive, and accurate detection of HIV-1 protease, in which fluorescent-labeled HIV-1 protease substrate peptide molecules were covalently linked to GO. In the absence of HIV-1 protease, fluorescein was effectively quenched by GO. In contrast, in the presence of HIV-1 protease, it would cleave the substrate peptide into short fragments, thus producing fluorescence. Based on this sensing strategy, HIV-1 protease could be detected at as low as 1.18 ng/mL. More importantly, the sensor could successfully detect HIV-1 protease in human serum. Such GO-based fluorescent sensors may find useful applications in many fields, including diagnosis of protease-related diseases, as well as sensitive and high-throughput screening of drug candidates. Graphical abstract ᅟ.
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