ArticleRNA biology2018
Global characterization of the Dicer-like protein DrnB roles in miRNA biogenesis in the social amoeba Dictyostelium discoideum.
Article in RNA biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed.
- RNA-guided RNA silencing by an Asgard archaeal Argonaute.Nature communications · 2024Article
- Chromosome-level genome assembly and annotation of the social amoeba Dictyostelium firmibasis.Scientific data · 2024Article
- Evolution of microRNAs in Amoebozoa and implications for the origin of multicellularity.Nucleic acids research · 2024Article
- Dicer structure and function: conserved and evolving features.EMBO reports · 2023Review
- Fractional 2'-O-methylation in the ribosomal RNA of Dictyostelium discoideum supports ribosome heterogeneity in Amoebozoa.Scientific reports · 2022Article
- Abundantly expressed class of noncoding RNAs conserved through the multicellular evolution of dictyostelid social amoebas.Genome research · 2021Article
- DIRS retrotransposons amplify via linear, single-stranded cDNA intermediates.Nucleic acids research · 2020Article
- Investigation of the host transcriptional response to intracellular bacterial infection using Dictyostelium discoideum as a host model.BMC genomics · 2019Article
Corrections and comments
- Updated byCorrection.2018
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Micro (mi)RNAs regulate gene expression in many eukaryotic organisms where they control diverse biological processes. Their biogenesis, from primary transcripts to mature miRNAs, have been extensively characterized in animals and plants, showing distinct differences between these phylogenetically distant groups of organisms. However, comparably little is known about miRNA biogenesis in organisms whose evolutionary position is placed in between plants and animals and/or in unicellular organisms. Here, we investigate miRNA maturation in the unicellular amoeba Dictyostelium discoideum, belonging to Amoebozoa, which branched out after plants but before animals. High-throughput sequencing of small RNAs and poly(A)-selected RNAs demonstrated that the Dicer-like protein DrnB is required, and essentially specific, for global miRNA maturation in D. discoideum. Our RNA-seq data also showed that longer miRNA transcripts, generally preceded by a T-rich putative promoter motif, accumulate in a drnB knock-out strain. For two model miRNAs we defined the transcriptional start sites (TSSs) of primary (pri)-miRNAs and showed that they carry the RNA polymerase II specific m
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