ArticleNature biomedical engineering2018
Cell-type-specific brain methylomes profiled via ultralow-input microfluidics.
Article in Nature biomedical engineering, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it.
- DNA methylation and histone modifications associated with antipsychotic treatment: a systematic review.Molecular psychiatry · 2025Pooled it
- The crosstalk between epigenetics and metabolism in the malignant cell.Discover oncology · 2026Review
- Genome-wide profiling of unmodified DNA using methyltransferase-directed tagging and enrichment.Cell reports methods · 2025Article
- Lossless Altered Histone Modification Analysis System (LAHMAS).Lab on a chip · 2025Article
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Microfluidic Biochips for Single-Cell Isolation and Single-Cell Analysis of Multiomics and Exosomes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Review
- Antipsychotic-induced epigenomic reorganization in frontal cortex of individuals with schizophrenia.eLife · 2024Article
- Advances in single-cell omics and multiomics for high-resolution molecular profiling.Experimental & molecular medicine · 2024Review
- Droplet-based bisulfite sequencing for high-throughput profiling of single-cell DNA methylomes.Nature communications · 2023Article
- Droplet-based bisulfite sequencing for high-throughput profiling of single-cell DNA methylomes.bioRxiv : the preprint server for biology · 2023Article
- Technologies and applications of single-cell DNA methylation sequencing.Theranostics · 2023Review
- Single-Cell DNA Methylation Analysis in Cancer.Cancers · 2022Review
- Temporally divergent regulatory mechanisms govern neuronal diversification and maturation in the mouse and marmoset neocortex.Nature neuroscience · 2022Article
- Microfluidic platforms for single neuron analysis.Materials today. Bio · 2022Review
- Introduction to Single-Cell DNA Methylation Profiling Methods.Biomolecules · 2021Review
- Single-cell analysis targeting the proteome.Nature reviews. Chemistry · 2020Review
- Microfluidic Platform for Next-Generation Sequencing Library Preparation with Low-Input Samples.Analytical chemistry · 2020Article
- Partitioning of hydrogels in 3D-printed microchannels.Lab on a chip · 2019Article
- Microfluidic epigenomic mapping technologies for precision medicine.Lab on a chip · 2019Review
- Mapping chromatin modifications at the single cell level.Development (Cambridge, England) · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
Methylomic analyses typically require substantial amounts of DNA, thus hindering studies involving scarce samples. Here, we show that microfluidic diffusion-based reduced representative bisulfite sequencing (MID-RRBS) permits high-quality methylomic profiling with nanogram-to-single-cell quantities of starting DNA. We used the microfluidic device, which allows for efficient bisulfite conversion with high DNA recovery, to analyse genome-wide DNA methylation in cell nuclei isolated from mouse brains and sorted into NeuN+ (primarily neuronal) and NeuN- (primarily glial) fractions, and to establish cell-type-specific methylomes. Genome-wide methylation and methylation in low-CpG-density promoter regions showed distinct patterns for NeuN+ and NeuN- fractions from the mouse cerebellum. The identification of substantial variations in the methylomic landscapes of the NeuN+ fraction of the frontal cortex of mice chronically treated with an atypical antipsychotic drug suggests that this technology can be broadly used for cell-type-specific drug profiling and for the study of drug-methylome interactions.
Identifiers
29963329PMC6023403What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.