ArticleMikrochimica acta2018
Fluorometric aptamer-based determination of ochratoxin A based on the use of graphene oxide and RNase H-aided amplification.
Article in Mikrochimica acta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 44 citations in OpenAlex.
- Article
- Recent trends in analysis of mycotoxins in food using carbon-based nanomaterials.Journal of food and drug analysis · 2022Review
- Nanomaterial-based aptamer biosensors for ochratoxin A detection: a review.Analytical and bioanalytical chemistry · 2022Review
- Aptamers-Diagnostic and Therapeutic Solution in SARS-CoV-2.International journal of molecular sciences · 2022Review
- Carbon-Based Nanocomposite Smart Sensors for the Rapid Detection of Mycotoxins.Nanomaterials (Basel, Switzerland) · 2021Review
- Electrochemical Biosensors in Food Safety: Challenges and Perspectives.Molecules (Basel, Switzerland) · 2021Review
- Advances in detection of infectious agents by aptamer-based technologies.Emerging microbes & infections · 2020Review
- Article
- A fluorometric aptamer-based assay for ochratoxin A by using exonuclease III-assisted recycling amplification.Mikrochimica acta · 2019Article
- A review on recent developments in optical and electrochemical aptamer-based assays for mycotoxins using advanced nanomaterials.Mikrochimica acta · 2019Review
- Fluorometric determination of RNase H via a DNAzyme conjugated to reduced graphene oxide, and its application to screening for inhibitors and activators.Mikrochimica acta · 2019Article
- Fluorescent Method for the Detection of Biothiols Using an Ag⁺-Mediated Conformational Switch.Sensors (Basel, Switzerland) · 2019Article
- Article
- Fluorometric determination of the activity of uracil-DNA glycosylase by using graphene oxide and exonuclease I assisted signal amplification.Mikrochimica acta · 2019Article
- A Label-Free Fluorescent Assay for the Rapid and Sensitive Detection of Adenosine Deaminase Activity and Inhibition.Sensors (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The authors describe a fluorometric assay for ochratoxin A (OTA) that is based on the use of graphene oxide and RNase H-aided amplification. On addition of OTA, cAPT is replaced from the APT/cAPT hybridization complex and then hybridizes with RNA labeled with a fluorophore at the 5'-end. Eventually, the fluorophore is released by RNase H cleavage. As the concentration of OTA increases, more cAPTs are displaced, this leading to fluorescence enhancement (best measured at excitation/emission wavelengths of 495/515 nm). This RNase H-assisted cycle response results in strong signal amplification. The limit of detection, calculated on the basis of a signal to noise ratio of 3, is 0.08 ng·mL
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