Evidence map›Paper›PMID 29961119›Full record

ArticleArchives of virology2018

Clinical evaluation of a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction assays for the detection of 16 respiratory viruses associated with community-acquired pneumonia.

Dan Zhang, Haiyan Mao, Xiuyu Lou, Junhang Pan, Hao Yan, Hongfeng Tang, Yan Shu, Yun Zhao, Xiaoli Cheng, Hong Tao and 2 more

Abstract readEvaluation Study
In one paragraph

Article in Archives of virology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Observational
  7. Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Dan ZhangKey Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Changping district, Beijing, 102206, China.
Haiyan MaoInstitute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China.
Xiuyu LouInstitute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China.
Junhang PanInstitute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China.
Hao YanInstitute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China.
Hongfeng TangDepartment of Pathology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310013, China.
Yan ShuDepartment of Pathology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310013, China.
Yun ZhaoDepartment of Pathology, Children's Hospital, Zhejiang University School of Medicine, Hangzhou, 310013, China.
Xiaoli ChengDepartment of Pathology, School of Basic Medicine, Hubei University of Medicine, Shiyan, Hubei, 442000, China.
Hong TaoDepartment of Rheumatology and Immunology Renmin Hospital, Hubei University of Medicine, Shiyan, 442000, Hubei, China.
Yanjun ZhangInstitute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, China. yjzhang@cdc.zj.cn.
Xuejun MaKey Laboratory for Medical Virology, National Health and Family Planning Commission, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, No. 155 Changbai Road, Changping district, Beijing, 102206, China. maxj@ivdc.chinacdc.cn.

Funding

Ministry of Science and Technology of the People's Republic of China 2016TFC1202700Ministry of Science and Technology of the People's Republic of China 2016YFC1200903Ministry of Science and Technology of the People's Republic of China 2017YFC1200503Ministry of Science and Technology of the People's Republic of China 2017ZX10104001Ministry of Science and Technology of the People's Republic of China 2017ZX10302301-004
6 · The paper itself

Abstract

We developed a panel of multiplex quantitative real-time reverse transcription polymerase chain reaction (mqRT-PCR) assay consisting of seven internally controlled qRT-PCR assays to detect 16 different respiratory viruses. We compared the new mqRT-PCR with a previously reported two-tube mRT-PCR assay using 363 clinical sputum specimens. The mqRT-PCR assay performed comparably with the two-tube assay for most viruses, offering the advantages of quantitative analysis, easier performance, lower susceptibility to contamination, and shorter turnaround time in laboratories equipped with conventional real-time PCR instrumentation, and it could therefore be a valuable tool for routine surveillance of respiratory virus infections in China.

Indexed as

Community-Acquired InfectionsHumansMultiplex Polymerase Chain ReactionPneumoniaReal-Time Polymerase Chain ReactionRespiratory Tract InfectionsViruses

Identifiers

PMID29961119
PMCPMC7087343

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.