Evidence map›Paper›PMID 29959790›Full record

ArticleJournal of clinical laboratory analysis2018

Development and evaluation of armored RNA-based standards for quantification of BCR-ABL1

Yu Fu, Rui Zhang, Qisheng Wu, Jiawei Zhang, Lihua Bao, Jinming Li

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 93% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Yu FuNational Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing, China.ORCID http://orcid.org/0000-0002-0253-8651
Rui ZhangNational Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing, China.
Qisheng WuNational Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing, China.
Jiawei ZhangNational Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing, China.
Lihua BaoDepartment of Nuclear Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.
Jinming LiNational Center for Clinical Laboratories, Beijing Hospital, National Center of Gerontology, Beijing, China.
Chinese Academy of Medical Sciences & Peking Union Medical College · CNBeijing Hospital · CNJiangsu Province Hospital · CN

Funding

Beijing Natural Science Foundation 7174345National Natural Science Foundation of China 81601848The Special Fund for Health Scientific Research in the Public Interest from National Population and Family Planning Commission of the People's Republic of China 201402018
6 · The paper itself

Abstract

backgroundStandards play an important role in detection of the BCR-ABL1 fusion gene (FG) transcript. However, the standards widely used in laboratories are mainly based on plasmids or cDNA, which cannot accurately reflect the process of RNA extraction and cDNA synthesis. Therefore, we aimed to develop armored RNA-based standards for p210 and p190 BCR-ABL1FG transcripts' quantification.

methodsUsing overlapping polymerase chain reaction (PCR) technology, we first linked a segment of the p210 or p190 BCR-ABL1FG transcript with four control genes (CGs; ABL1, BCR, GUSB, and B2M) to form p210FG-CG and p190FG-CG. Subsequently, using armored RNA technology, we prepared p210FG-CG- and p190FG-CG-armored RNAs and the p210FG-CG and p190FG-CG standards, the values of which were assigned by digital PCR (dPCR).

resultsThe p210FG-CG and p190FG-CG standards were stable and homogeneous, and were significantly linear with r

conclusionThe overall variability of detection of BCR-ABL1 transcripts decreased significantly when using the p210FG-CG or p190FG-CG standards, especially the p190FG-CG standard.

Indexed as

Fusion Proteins, bcr-ablHumansNormal DistributionPolymerase Chain ReactionReference ValuesRNARNA, MessengerFusion Proteins, bcr-ablRNARNA, Messengerarmored RNABCR-ABL1leukemiareverse transcription-quantitative polymerase chain reactionstandard

Identifiers

PMID29959790
PMCPMC6816845
OpenAlexW2809916913

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.