In Vitro System for Coupling RNAP II Transcription to Primary microRNA Processing and a Three-Way System for RNAP II Transcription/Splicing/microRNA Processing.

Shanye Yin, Alexander Iocolano, Yong Yu, Jaya Gangopadhyay, Robin Reed

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

SF3B1 homeostasis is critical for survival and therapeutic response in T cell leukemia.Science advances · 2022
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shanye YinDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA.

Alexander IocolanoDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA.

Yong YuDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA.

Jaya GangopadhyayDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA.

Robin ReedDepartment of Cell Biology, Harvard Medical School, Boston, MA, USA. robin_reed@hms.harvard.edu.

Funding

RNA Processing Machines in Biology and DiseaseR35GM122524 · NIGMS · HARVARD MEDICAL SCHOOL · PI MOAZED, DANESH · 2017 to 2023

$4.1M

NIGMS NIH HHS R35 GM122524

6 · The paper itself

Abstract

In the genome, primary microRNAs (pri-miRNAs) are encoded either as independent transcriptional units with their own promoters (intergenic miRNAs) or within the introns of other genes (intronic miRNAs). Here, we report two methods, one that we established for coupled RNAP II transcription and pri-miRNA processing and the other that is a three-way system for RNAP II transcription, pri-miRNA processing, and pre-mRNA splicing. In these systems, CMV-DNA constructs encoding the processing substrates are incubated in HeLa cell nuclear extracts in the presence of

Indexed as

RNA SplicingTranscription, GeneticCell-Free SystemHeLa CellsHumansMicroRNAsRNA Polymerase IIRNA PrecursorsMicroRNAsRNA Polymerase IIRNA PrecursorsCoupled steps in gene expressionPre-mRNA splicingPrimary microRNA processingRNAP II transcription

Identifiers

PMID29959672

DOI10.1007/978-1-4939-8624-8_4

PMCPMC6346423

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.