ArticlePlant molecular biology2018
Concurrent modifications in the three homeologs of Ms45 gene with CRISPR-Cas9 lead to rapid generation of male sterile bread wheat (Triticum aestivum L.).
Article in Plant molecular biology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 112 citations in OpenAlex.
- Wheat Biofortification for Enhancing Iron, Zinc, and Protein: The Role of Mutation Breeding.Plants (Basel, Switzerland) · 2026Review
- TaRIP2 Positively Regulates Wheat Pollen Wall Formation Through MYB80-Controlled Lipid Metabolism.Plant biotechnology journal · 2026Article
- Optimization of functional genetics tools for a model tetraploid Capsella bursa-pastoris, with focus on homoeolog-aware gene editing.Transgenic research · 2026Article
- Challenges and Opportunities with CRISPR-Based Genome Editing in Legume Crops.Functional & integrative genomics · 2026Review
- CRISPR-Cas technologies for precision genome editing in plants: advances, applications, and future perspectives.Frontiers in plant science · 2026Review
- New genomic resources to boost research in reproductive biology to enable cost-effective hybrid seed production.The plant genome · 2025Article
- Exploring the Strategies of Male Sterility for Hybrid Development in Hexaploid Wheat: Prevailing Methods and Potential Approaches.Rice (New York, N.Y.) · 2025Review
- A TOPLESS-related gene regulates both male and female sterility in wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Abiotic stress responses in forage crops and grasses: the role of secondary metabolites and biotechnological interventions.Frontiers in plant science · 2025Review
- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- Targeted genome-modification tools and their advanced applications in crop breeding.Nature reviews. Genetics · 2024Review
- Article
- Harnessing the potential of mutation breeding, CRISPR genome editing, and beyond for sustainable agriculture.Functional & integrative genomics · 2024Review
- Putting CRISPR-Cas system in action: a golden window for efficient and precise genome editing for crop improvement.GM crops & food · 2023Review
- Establishment of new convenient two-line system for hybrid production by targeting mutation ofHorticulture research · 2023Article
- CRISPR/Cas Technology Revolutionizes Crop Breeding.Plants (Basel, Switzerland) · 2023Review
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- Review
- Targeted mutagenesis with sequence-specific nucleases for accelerated improvement of polyploid crops: Progress, challenges, and prospects.The plant genome · 2023Review
- CRISPR/Cas9-targeted mutagenesis of TaDCL4, TaDCL5 and TaRDR6 induces male sterility in common wheat.Plant biotechnology journal · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageHexaploid bread wheat is not readily amenable to traditional mutagenesis approaches. In this study, we show efficient utilization of CRISPR-Cas system and Next Generation Sequencing for mutant analysis in wheat. Identification and manipulation of male fertility genes in hexaploid bread wheat is important for understanding the molecular basis of pollen development and to obtain novel sources of nuclear genetic male sterility (NGMS). The maize Male sterile 45 (Ms45) gene encodes a strictosidine synthase-like enzyme and has been shown to be required for male fertility. To investigate the role of Ms45 gene in wheat, mutations in the A, B and D homeologs were produced using CRISPR-Cas9. A variety of mutations in the three homeologs were recovered, including a plant from two different genotypes each with mutations in all three homeologs. Genetic analysis of the mutations demonstrated that all three wheat Ms45 homeologs contribute to male fertility and that triple homozygous mutants are required to abort pollen development and achieve male sterility. Further, it was demonstrated that a wild-type copy of Ms45 gene from rice was able to restore fertility to these wheat mutant plants. Taken together, these observations provide insights into the conservation of MS45 function in a polyploid species. Ms45 based NGMS can be potentially utilized for a Seed Production Technology (SPT)-like hybrid seed production system in wheat.
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