ArticleScientific reports2018
Differential preservation of endogenous human and microbial DNA in dental calculus and dentin.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 128 citations in OpenAlex.
- Comparing the Performance of Double-Stranded and Single-Stranded DNA Libraries for Ancient Oral Microbiome Reconstruction.Molecular ecology resources · 2026Article
- The Oral Microbiome of King Richard III of England.American journal of biological anthropology · 2026Article
- Lifestyle Impacts the Oral Microbiome of Classical and Post-Classical Societies in Italy.American journal of biological anthropology · 2026Article
- Interactions at theMicrobiology (Reading, England) · 2026Review
- Dental Calculus Formation Is Linked to Diet and Phylogeny in Mammals.Ecology and evolution · 2026Article
- Ancient Species Diversity and Niche Adaptation in Tannerella and Porphyromonas Revealed Through Pangenomics.Genome biology and evolution · 2026Article
- Prokaryotic assemblages recovered by sedimentary DNA record natural and human-driven disturbances over the past 13 500 years in a cultural landscape.The ISME journal · 2026Article
- Thermal desorption GC/MS on human dental calculus detected minute millet consumption in medieval Ukraine.Scientific reports · 2025Article
- Host Traits Impact the Outcome of Metagenomic Library Preparation From Dental Calculus Samples Across Diverse Mammals.Molecular ecology resources · 2025Article
- Wet Lab Protocols Matter: Choice of DNA Extraction and Library Preparation Protocols Bias Ancient Oral Microbiome Recovery.Molecular ecology resources · 2025Article
- Ancient DNA and biomarkers from artefacts: insights into technology and cultural practices in Neolithic Europe.Proceedings. Biological sciences · 2025Article
- Bone Adhered Sediments as a Source of Target and Environmental DNA and Proteins.Molecular biology and evolution · 2025Article
- Article
- Review
- Streptococcus abundance and oral site tropism in humans and non-human primates reflects host and lifestyle differences.NPJ biofilms and microbiomes · 2025Article
- Exploring the potential of dental calculus to shed light on past human migrations in Oceania.Nature communications · 2024Article
- Towards predicting the geographical origin of ancient samples with metagenomic data.Scientific reports · 2024Article
- Metagenomic and paleopathological analyses of a historic documented collection explore ancient dental calculus as a diagnostic tool.Scientific reports · 2024Article
- A Journey into the Evolution of Human Host-Oral Microbiome Relationship through Ancient Dental Calculus: A Scoping Review.Microorganisms · 2024Article
- Ancient Genomes From Bronze Age Remains Reveal Deep Diversity and Recent Adaptive Episodes for Human Oral Pathobionts.Molecular biology and evolution · 2024Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
21 authors at 9 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dental calculus (calcified dental plaque) is prevalent in archaeological skeletal collections and is a rich source of oral microbiome and host-derived ancient biomolecules. Recently, it has been proposed that dental calculus may provide a more robust environment for DNA preservation than other skeletal remains, but this has not been systematically tested. In this study, shotgun-sequenced data from paired dental calculus and dentin samples from 48 globally distributed individuals are compared using a metagenomic approach. Overall, we find DNA from dental calculus is consistently more abundant and less contaminated than DNA from dentin. The majority of DNA in dental calculus is microbial and originates from the oral microbiome; however, a small but consistent proportion of DNA (mean 0.08 ± 0.08%, range 0.007-0.47%) derives from the host genome. Host DNA content within dentin is variable (mean 13.70 ± 18.62%, range 0.003-70.14%), and for a subset of dentin samples (15.21%), oral bacteria contribute > 20% of total DNA. Human DNA in dental calculus is highly fragmented, and is consistently shorter than both microbial DNA in dental calculus and human DNA in paired dentin samples. Finally, we find that microbial DNA fragmentation patterns are associated with guanine-cytosine (GC) content, but not aspects of cellular structure.
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