Evidence map›Paper›PMID 29959254›Full record

ArticleApplied and environmental microbiology2018

High-Throughput Microfluidic Sorting of Live Magnetotactic Bacteria.

Andy Tay, Daniel Pfeiffer, Kathryn Rowe, Aaron Tannenbaum, Felix Popp, Robert Strangeway, Dirk Schüler, Dino Di Carlo

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Isolation Techniques of Micro/Nano-Scaled Species for Biomedical Applications.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. Article
  8. Review
  9. Cell Separations and Sorting.Analytical chemistry · 2020
    Review
  10. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Andy TayDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, California, USA.
Daniel PfeifferDepartment of Microbiology, University of Bayreuth, Bayreuth, Germany.
Kathryn RoweInstitute of Geophysics and Planetary Physics, University of California, Los Angeles, Los Angeles, California, USA.
Aaron TannenbaumInstitute of Geophysics and Planetary Physics, University of California, Los Angeles, Los Angeles, California, USA.
Felix PoppDepartment of Microbiology, University of Bayreuth, Bayreuth, Germany.
Robert StrangewayInstitute of Geophysics and Planetary Physics, University of California, Los Angeles, Los Angeles, California, USA.
Dirk SchülerDepartment of Microbiology, University of Bayreuth, Bayreuth, Germany.
Dino Di CarloDepartment of Bioengineering, University of California, Los Angeles, Los Angeles, California, USA dicarlo@ucla.edu.

Funding

Engineering the Intracellular Micro- and NanoenvironmentDP2OD007113 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI DI CARLO, DINO · 2010 to 2010
$2.3M
HIGH-END CRYOEM INSTRUMENT FOR UCLA ELECTRON IMAGING CTR FOR NANOMACHINES: AIDSS10RR023057 · NCRR · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2006 to 2006
$1.6M
NCRR NIH HHS S10 RR023057NIH HHS DP2 OD007113
6 · The paper itself

Abstract

Magnetic nanoparticles (MNPs) are useful for many biomedical applications, but it is challenging to synthetically produce them in large numbers with uniform properties and surface functionalization. Magnetotactic bacteria (MTB) produce magnetosomes with homogenous sizes, shapes, and magnetic properties. Consequently, there is interest in using MTB as biological factories for MNP production. Nonetheless, MTB can only be grown to low yields, and wild-type strains produce low numbers of MNPs/bacterium. There are also limited technologies to facilitate the selection of MTB with different magnetic contents, such as MTB with compromised and enhanced biomineralization ability. Here, we describe a magnetic microfluidic platform combined with transient cold/alkaline treatment to temporarily reduce the rapid flagellar motion of MTB without compromising their long-term proliferation and biomineralization ability for separating MTB on the basis of their magnetic contents. This strategy enables live MTB to be enriched, which, to the best of our knowledge, has not been achieved with another previously described magnetic microfluidic device that makes use of ferrofluid and heat. Our device also facilitates the high-throughput (25,000 cells/min) separation of wild-type

Indexed as

High-Throughput Screening AssaysMagnetite NanoparticlesMagnetosomesMagnetospirillumMicrofluidic Analytical TechniquesMicrofluidicsMagnetite Nanoparticlesenrichmentmagnetic nanoparticlesmagnetotactic bacteriamicrofluidic

Identifiers

PMID29959254
PMCPMC6102981

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.