ReviewBiophysical journal2018
Recent Developments in Single-Cell RNA-Seq of Microorganisms.
Review in Biophysical journal, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Altered molecular signature of the intestinal microbiota in irritable bowel syndrome patients versus healthy controls: An updated systematic review.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2026Pooled it
- Single-bacterium metabolome revealing heterogeneous cellular states in bacterial populations.Nature communications · 2026Article
- A Brief Review of Microbial Omics: Methods and Perspectives.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Article
- Bioinformatics perspectives on transcriptomics: A comprehensive review of bulk and single-cell RNA sequencing analyses.Quantitative biology (Beijing, China) · 2025Review
- Advanced genetic techniques in fungal pathogen research.mSphere · 2024Review
- Single-Molecule Fluorescent In Situ Hybridization (smFISH) for RNA Detection in Bacteria.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Bacterial single-cell transcriptomics: Recent technical advances and future applications in dentistry.The Japanese dental science review · 2023Review
- Article
- Article
- mDrop-Seq: Massively Parallel Single-Cell RNA-Seq ofVaccines · 2021Article
- Single-Cell Technologies to Study Phenotypic Heterogeneity and Bacterial Persisters.Microorganisms · 2021Review
- Droplet microfluidics on analysis of pathogenic microbes for wastewater-based epidemiology.Trends in analytical chemistry : TRAC · 2021Review
- Single-cell RNA sequencing in cancer: Applications, advances, and emerging challenges.Molecular therapy oncolytics · 2021Review
- Evolution of Drug-ResistantFrontiers in microbiology · 2021Review
- Microbial single-cell omics: the crux of the matter.Applied microbiology and biotechnology · 2020Review
- How bacteria recognise and respond to surface contact.FEMS microbiology reviews · 2020Review
- Analysis of themSphere · 2019Article
- The dark side of technological advances in analysis of microbial ecosystems.Journal of animal science and biotechnology · 2019Review
- Single-Cell RNA Sequencing of Plant-Associated Bacterial Communities.Frontiers in microbiology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single-cell transcriptome analysis through next-generation sequencing (single-cell RNA-seq) has been used broadly to address important biological questions. It has proved to be very powerful, and many exciting new biological discoveries have been achieved in the last decade. Its application has greatly improved our understanding of diverse biological processes and the underlying molecular mechanisms, an understanding that would not have been achievable by conventional analysis based on bulk populations. However, so far, single-cell RNA-seq analysis has been used mostly for higher organisms. For microorganisms, single-cell RNA-seq has not been widely used, mainly because the stiff cell wall prevents effective lysis, much less starting RNA material is obtained, and the RNA lacks polyadenylated tails for universal priming of mRNA molecules. In general, the detection efficiency of current single-cell RNA-seq technologies is very low, and further development or improvement of these technologies is required for exploring the microbial world at single-cell resolution. Here, we briefly review recent developments in single-cell RNA-seq of microorganisms and discuss current challenges and future directions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.