Evidence map›Paper›PMID 29958103›Full record

ArticleCell2018

Size-Dependent Segregation Controls Macrophage Phagocytosis of Antibody-Opsonized Targets.

Matthew H Bakalar, Aaron M Joffe, Eva M Schmid, Sungmin Son, Marija Podolski, Daniel A Fletcher

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 103 papers.

0numbers the graph read from it
0cells of the map it votes in
103citing papers in PubMed
11.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

103 citing papers in PubMed, 164 citations in OpenAlex.

  1. Review
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  11. EPY001, a Novel Monoclonal Antibody AgainstInternational journal of molecular sciences · 2025
    Article
  12. Functional Differences Between SIRPα Splice Isoforms.Genes to cells : devoted to molecular & cellular mechanisms · 2025
    Article
  13. Article
  14. Article
  15. Clustering of Membrane Receptors: Insights from DNA Origami-Based Approaches.Small (Weinheim an der Bergstrasse, Germany) · 2025
    Review
  16. Article
  17. Article
  18. Review
  19. Article
  20. Article

43 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 1 country.

Matthew H BakalarDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; UC Berkeley/UC San Francisco Graduate Group in Bioengineering, Berkeley, CA 94720, USA.
Aaron M JoffeDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; UC Berkeley/UC San Francisco Graduate Group in Bioengineering, Berkeley, CA 94720, USA.
Eva M SchmidDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Sungmin SonDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Marija PodolskiDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA.
Daniel A FletcherDepartment of Bioengineering, University of California, Berkeley, Berkeley, CA 94720, USA; UC Berkeley/UC San Francisco Graduate Group in Bioengineering, Berkeley, CA 94720, USA; Division of Biological Systems and Engineering, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Chan Zuckerberg Biohub, San Francisco, CA 94158, USA. Electronic address: fletch@berkeley.edu.
University of California, Berkeley · USUniversity of California, San Francisco · USLawrence Berkeley National Laboratory · US

Funding

Force-Mediated Membrane FusionR01GM114671 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI FLETCHER, DANIEL A · 2016 to 2019
$1.4M
NIGMS NIH HHS R01 GM114671
6 · The paper itself

Abstract

Macrophages protect the body from damage and disease by targeting antibody-opsonized cells for phagocytosis. Though antibodies can be raised against antigens with diverse structures, shapes, and sizes, it is unclear why some are more effective at triggering immune responses than others. Here, we define an antigen height threshold that regulates phagocytosis of both engineered and cancer-specific antigens by macrophages. Using a reconstituted model of antibody-opsonized target cells, we find that phagocytosis is dramatically impaired for antigens that position antibodies >10 nm from the target surface. Decreasing antigen height drives segregation of antibody-bound Fc receptors from the inhibitory phosphatase CD45 in an integrin-independent manner, triggering Fc receptor phosphorylation and promoting phagocytosis. Our work shows that close contact between macrophage and target is a requirement for efficient phagocytosis, suggesting that therapeutic antibodies should target short antigens in order to trigger Fc receptor activation through size-dependent physical segregation.

Indexed as

PhagocytosisAnimalsAntibodies, MonoclonalAntigensCarcinoembryonic AntigenGene EditingIntegrinsLeukocyte Common AntigensMacrophagesMiceOpsonin ProteinsPhosphorylationRAW 264.7 CellsReceptors, FcUnilamellar LiposomesAntibodies, MonoclonalAntigensCarcinoembryonic AntigenIntegrinsLeukocyte Common AntigensOpsonin ProteinsReceptors, FcUnilamellar Liposomesantibodyantigencancer immunotherapyCD45Fc receptorimmune modulationITAMkinetic segregation modelmacrophagesphagocytosisprotein segregation

Identifiers

PMID29958103
PMCPMC6067926
OpenAlexW2794833512

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.