ArticleScientific reports2018
Live cell imaging reveals focal adhesions mechanoresponses in mammary epithelial cells under sustained equibiaxial stress.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 28 citations in OpenAlex.
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- Stiff Extracellular Matrix Promotes Invasive Behaviors of Trophoblast Cells.Bioengineering (Basel, Switzerland) · 2023Article
- Contribution of mechanical homeostasis to epithelial-mesenchymal transition.Cellular oncology (Dordrecht, Netherlands) · 2022Review
- Strain-induced mechanoresponse depends on cell contractility and BAG3-mediated autophagy.Molecular biology of the cell · 2021Article
- Condensation tendency and planar isotropic actin gradient induce radial alignment in confined monolayers.eLife · 2021Article
- Focus on time: dynamic imaging reveals stretch-dependent cell relaxation and nuclear deformation.Biophysical journal · 2021Article
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- Molecular Regulators of Cellular Mechanoadaptation at Cell-Material Interfaces.Frontiers in bioengineering and biotechnology · 2020Review
- Improving Quality, Reproducibility, and Usability of FRET-Based Tension Sensors.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2019Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Mechanical stimuli play a key role in many cell functions such as proliferation, differentiation and migration. In the mammary gland, mechanical signals such as the distension of mammary epithelial cells due to udder filling are proposed to be directly involved during lactation and involution. However, the evolution of focal adhesions -specialized multiprotein complexes that mechanically connect cells with the extracellular matrix- during the mammary gland development, as well as the influence of the mechanical stimuli involved, remains unclear. Here we present the use of an equibiaxial stretching device for exerting a sustained normal strain to mammary epithelial cells while quantitatively assessing cell responses by fluorescence imaging techniques. Using this approach, we explored changes in focal adhesion dynamics in HC11 mammary cells in response to a mechanical sustained stress, which resembles the physiological stimuli. We studied the relationship between a global stress and focal adhesion assembly/disassembly, observing an enhanced persistency of focal adhesions under strain as well as an increase in their size. At a molecular level, we evaluated the mechanoresponses of vinculin and zyxin, two focal adhesion proteins postulated as mechanosensors, observing an increment in vinculin molecular tension and a slower zyxin dynamics while increasing the applied normal strain.
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