Evidence map›Paper›PMID 29954457›Full record

ArticleStem cell research & therapy2018

Human mesenchymal stem cell-derived extracellular vesicles/estrogen combined therapy safely ameliorates experimentally induced intrauterine adhesions in a female rat model.

Nesrine Ebrahim, Ola Mostafa, Rania Ebrahim El Dosoky, Inas A Ahmed, Ahmed S Saad, Abeer Mostafa, Dina Sabry, Khalid Abdelaziz Ibrahim, Ayman Samir Farid

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed, 3 pooled it
7.9field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 3 syntheses or guidelines pooled it, 110 citations in OpenAlex.

  1. Pooled it
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  3. New advances in the treatment of thin endometrium.Frontiers in endocrinology · 2024
    Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Nesrine EbrahimDepartment of Histology and Cell Biology, Faculty of Medicine, Benha University, Banha, 13518, Qalyubia, Egypt.
Ola MostafaDepartment of Histology and Cell Biology, Faculty of Medicine, Benha University, Banha, 13518, Qalyubia, Egypt.
Rania Ebrahim El DosokyDepartment of Histology and Cell Biology, Faculty of Medicine, Benha University, Banha, 13518, Qalyubia, Egypt.
Inas A AhmedDepartment of Medical Biochemistry, Faculty of Medicine, Benha University, Banha, 13518, Qalyubia, Egypt.
Ahmed S SaadDepartment of Obstetrics and Gynecology, Faculty of Medicine, Benha University, Banha, 13518, Qalyubia, Egypt.
Abeer MostafaDepartment of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
Dina SabryDepartment of Medical Biochemistry, Faculty of Medicine, Cairo University, Cairo, 11562, Egypt.
Khalid Abdelaziz IbrahimDepartment of Obstetrics and Gynecology, Faculty of Medicine, Benha University, Banha, 13518, Qalyubia, Egypt.
Ayman Samir FaridDepartment of Clinical Pathology, Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh, 13736, Qalyubia, Egypt. ayman.samir@fvtm.bu.edu.eg.ORCID 0000-0002-7844-7967
Benha University · EGCairo University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMesenchymal stem cells (MSCs) have diverse functions in regulating injury and inflammation through the secretion of extracellular vesicles (EVs).

methodsIn this study, we investigated the systemic administration of extracellular vesicles derived from human umbilical cord mesenchymal stem cells (UCMSCs-EVs) as a therapeutic agent for intrauterine adhesions (IUAs) caused by endometrial injury. Additionally, we investigated the therapeutic impact of both UCMSCs-EVs and estrogen either separately or in combination in a rat model. The inflammation, vascularization, proliferation, and extent of fibrosis were assessed by a histopathological and immunohistochemical assessment using transforming growth factor (TGF)-β as a fibrotic marker and vascular endothelial growth factor (VEGF) as a vascular marker. Additionally, quantitative real-time polymerase chain reaction (qRT-PCR) was used to analyze the expression of tumor necrosis factor (TNF)-α, interleukin (IL)-1, IL-6 (inflammatory cytokines), CD140b (a marker of endometrial stem cells), and RUNX2 (an antifibrotic factor). Finally, Western blotting was used to evaluate collagen I and β-actin expression.

resultsThe therapeutic groups treated with either UCMSCs-EVs alone or combined with estrogen exhibited a significant decrease in inflammation and fibrosis (TNF-α, TGF-β, IL-1, IL-6, RUNX2, and collagen-I) as well as a significant decrease in vascularization (VEGF) compared with the untreated rats with IUAs. The most significant results were obtained in animals with IUAs that received a combined therapy of UCMSCs-EVs and estrogen.

conclusionsWe conclude that the synergistic action of human UCMSCs-EVs combined with estrogen provides a highly effective alternative regenerative agent in IUA treatment.

Indexed as

AnimalsDisease Models, AnimalExtracellular VesiclesFemaleHumansMesenchymal Stem CellsRatsTissue AdhesionsCollagenEstrogenIL-1IL-6Intrauterine adhesionsRUNX2TGF-βTNF-αUCMSCs-EVs

Identifiers

PMID29954457
PMCPMC6027762
OpenAlexW2811131800

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.