ArticleCell cycle (Georgetown, Tex.)2018
Monitoring dimeric status of IZUMO1 during the acrosome reaction in living spermatozoon.
Article in Cell cycle (Georgetown, Tex.), 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 22 citations in OpenAlex.
- The Role of Sperm Proteins IZUMO1 and TMEM95 in Mammalian Fertilization: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Sperm surface protein disulfide isomerase ERp57 is crucial for mammalian fertilization but functions independently of IZUMO1.Cellular & molecular biology letters · 2026Article
- JAGGED1 modulates bull spermatozoa acrosome reaction and fertilization.Reproduction & fertility · 2026Article
- Membrane Fusions During Mammalian Fertilization.Advances in experimental medicine and biology · 2026Review
- JUNO-coated beads as a functional assay to capture and characterize fertilization-competent human sperm.Human reproduction open · 2026Article
- Review
- Molecular mechanisms leading to gamete fusion.Fukushima journal of medical science · 2025Review
- IZUMO1 Receptor Localization during Hyaluronic Acid Selection in Human Spermatozoa.Biomedicines · 2023Article
- A Frame-by-Frame Glance at Membrane Fusion Mechanisms: From Viral Infections to Fertilization.Biomolecules · 2023Review
- New insights into posttranslational modifications of proteins during bull sperm capacitation.Cell communication and signaling : CCS · 2023Article
- Human sperm TMEM95 binds eggs and facilitates membrane fusion.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- C11orf94/Frey is a key regulator for male fertility by controlling Izumo1 complex assembly.Science advances · 2022Article
- Mysteries and unsolved problems of mammalian fertilization and related topics.Biology of reproduction · 2022Review
- Partial Sperm beta1 Integrin Subunit Deletion Proves its Involvement in Mouse Gamete Adhesion/Fusion.International journal of molecular sciences · 2020Article
- Sperm SPACA6 protein is required for mammalian Sperm-Egg Adhesion/Fusion.Scientific reports · 2020Article
- Phosphatidylserine on viable sperm and phagocytic machinery in oocytes regulate mammalian fertilization.Nature communications · 2019Article
- Sperm IZUMO1-Dependent Gamete Fusion Influences Male Fertility in Mice.International journal of molecular sciences · 2019Article
- Integrated Analyses of Phenotype and Quantitative Proteome of CMTM4 Deficient Mice Reveal Its Association with Male Fertility.Molecular & cellular proteomics : MCP · 2019Article
- Alternative splicing of the Izumo1 gene ensures triggering gamete fusion in mice.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The acrosome reaction (AR) is indispensable for successful spermatozoon-oocyte fusion. Recent studies have indicated that sperm IZUMO1 gradually gathers in the equatorial segment (EQ), which is the initiation site of sperm-egg fusion, after the AR. In addition, by examining the binding process of oocytes and Izumo1-expressing cultured cells to reconstitute the early steps of fertilization, we previously demonstrated that robust IZUMO1-dependent adhesion specifically occurs at the contact site along with the dimerization of IZUMO1. However, when IZUMO1 dimerizes after the AR in living spermatozoon is unknown. Here, we report dynamics of IZUMO1 dimerization during the AR in spermatozoa by combining transgenic mice and time-lapse imaging using a set of bimolecular fluorescence complementation (BiFC) probes. Surprisingly, dimeric IZUMO1 was already formed at the acrosomal cap region before the AR and redistributed into the EQ after the AR. We categorized the translocation of the dimer into two types: Type 1, the near-simultaneous appearance of BiFC signals with IZUMO1-mCherry; and Type 2, the delayed formation of dimer in the EQ. Those findings suggest that, before encountering oocytes, spermatozoa are prepared to boost their affinity with JUNO.
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