ArticlePloS one2018
Four-dimensional analysis by high-speed holographic imaging reveals a chiral memory of sperm flagella.
Article in PloS one, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- RGS22 is a metazoa-specific radial spoke component required for coordinated ciliary beating.Nature communications · 2026Article
- Precise 3D Tracking of Highly Non-Planar Eukaryotic Flagellar Beating Patterns Using Digital Holographic Microscopy.Small methods · 2026Article
- Coordinated and compartmentalized functions of CAII and CAIV establish a dual pH-regulatory mechanism essential for sperm maturation and capacitation.bioRxiv : the preprint server for biology · 2025Article
- Human sperm rotate with a conserved direction during free swimming in four dimensions.Journal of cell science · 2023Article
- CatSper Calcium Channels: 20 Years On.Physiology (Bethesda, Md.) · 2023Review
- In situ cryo-electron tomography reveals the asymmetric architecture of mammalian sperm axonemes.Nature structural & molecular biology · 2023Article
- cAMP and the Fibrous Sheath Protein CABYR (CaInternational journal of molecular sciences · 2022Article
- 3D structure and in situ arrangements of CatSper channel in the sperm flagellum.Nature communications · 2022Article
- CatSper and its CaM-like CaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Multifocal imaging for precise, label-free tracking of fast biological processes in 3D.Nature communications · 2021Article
- Reconstruction of the three-dimensional beat pattern underlying swimming behaviors of sperm.The European physical journal. E, Soft matter · 2021Article
- Micromotor-mediated sperm constrictions for improved swimming performance.The European physical journal. E, Soft matter · 2021Article
- Flagellar energetics from high-resolution imaging of beating patterns in tethered mouse sperm.eLife · 2021Article
- Modelling Motility: The Mathematics of Spermatozoa.Frontiers in cell and developmental biology · 2021Review
- Exploiting the potential of commercial digital holographic microscopy by combining it with 3D matrix cell culture assays.Scientific reports · 2020Article
- Hitting the wall: Human sperm velocity recovery under ultra-confined conditions.Biomicrofluidics · 2020Article
- Rotational motion and rheotaxis of human sperm do not require functional CatSper channels and transmembrane CaThe EMBO journal · 2020Article
- Motility Analysis of Trypanosomatids.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Single-cell Motility Analysis of Tethered Human Spermatozoa.Bio-protocol · 2019Article
- Correction: Four-dimensional analysis by high-speed holographic imaging reveals a chiral memory of sperm flagella.PloS one · 2018Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here high-speed Digital Holographic Microscopy (DHM) records sperm flagellar waveforms and swimming paths in 4 dimensions (X, Z, and t). We find flagellar excursions into the Z-plane nearly as large as the envelope of the flagellar waveform projected onto the XY-plane. These Z-plane excursions travel as waves down the flagellum each beat cycle. DHM also tracks the heads of free-swimming sperm and the dynamics and chirality of rolling of sperm around their long axis. We find that mouse sperm roll CW at the maximum positive Z-plane excursion of the head, then roll CCW at the subsequent maximum negative Z-plane excursion. This alternating chirality of rolling indicates sperm have a chiral memory. Procrustes alignments of path trajectories for sequences of roll-counterroll cycles show that path chirality is always CW for the cells analyzed in this study. Human and bull sperm lack distinguishable left and right surfaces, but DHM still indicates coordination of Z-plane excursions and rolling events. We propose that sperm have a chiral memory that resides in a hypothetical elastic linkage within the flagellar machinery, which stores some of the torque required for a CW or CCW roll to reuse in the following counter-roll. Separate mechanisms control path chirality.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.