ArticleMolecular biology of the cell2018
A beginner's guide to rigor and reproducibility in fluorescence imaging experiments.
Article in Molecular biology of the cell, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
48 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Prevalence and practices of immunofluorescent cell image processing: a systematic review.Frontiers in cellular neuroscience · 2023Pooled it
- Cellular Characterization Using Microwave-Assisted Immunocytochemistry, A Time-Efficient Alternative to Traditional Immunocytochemistry.Current protocols · 2026Article
- BlinkFusion: modular platform quantifying labeling efficiency and photophysics in regular and super-resolution fluorescence microscopy.Frontiers in bioinformatics · 2026Article
- There and back again: a cell biologist's journey from organelles to molecules.Biological chemistry · 2025Review
- AnEnvironmental science & technology · 2025Article
- Best practices in plant fluorescence imaging and reporting: A primer.The Plant cell · 2025Review
- Geometrically constrained cytoskeletal reorganisation modulates DNA nanostructures uptake.Journal of materials chemistry. B · 2025Article
- A murine model ofMicrobiology spectrum · 2025Article
- Article
- Peptide-coated DNA nanostructures as a platform for control of lysosomal function in cells.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2024Article
- Exploring the influence of silicon oxide microchips shape on cellular uptake using imaging flow cytometry.Mikrochimica acta · 2024Article
- A murine model ofbioRxiv : the preprint server for biology · 2024Article
- DCM-Spheroid Morphs Express PADs and Citrullinated Cytoskeletal Proteins.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2024Article
- Validation of a semi-quantitative scoring system and workflow for analysis of fluorescence quantification in companion animals.Frontiers in veterinary science · 2024Article
- Cell Cycle Mapping Using Multiplexed Immunofluorescence.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Iron oxide nanoparticles trigger endoplasmic reticulum damage in steatotic hepatic cells.Nanoscale advances · 2023Article
- Sensitivity of endogenous autofluorescence in HeLa cells to the application of external magnetic fields.Scientific reports · 2023Article
- Mechanical Regulation of Mitochondrial Dynamics and Function in a 3D-Engineered Liver Tumor Microenvironment.ACS biomaterials science & engineering · 2023Article
- Tissue-Like 3D Standard and Protocols for Microscope Quality Management.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2023Article
- Virus-free transfection, transient expression, and purification of human cardiac myosin in mammalian muscle cells for biochemical and biophysical assays.Scientific reports · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Fluorescence light microscopy is an indispensable approach for the investigation of cell biological mechanisms. With the development of cutting-edge tools such as genetically encoded fluorescent proteins and superresolution methods, light microscopy is more powerful than ever at providing insight into a broad range of phenomena, from bacterial fission to cancer metastasis. However, as with all experimental approaches, care must be taken to ensure reliable and reproducible data collection, analysis, and reporting. Each step of every imaging experiment, from design to execution to communication to data management, should be critically assessed for bias, rigor, and reproducibility. This Perspective provides a basic "best practices" guide for designing and executing fluorescence imaging experiments, with the goal of introducing researchers to concepts that will help empower them to acquire images with rigor.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.