ArticleOncoTargets and therapy2018
Impact of RNA integrity and blood sample storage conditions on the gene expression analysis.
Article in OncoTargets and therapy, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 79 citations in OpenAlex.
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- Development of an End-to-End Total RNA Sequencing Quality Control Framework for Blood-Based Biomarker Discovery.The Journal of molecular diagnostics : JMD · 2025Article
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- Extraction of high quality and high yield RNA from frozen EDTA blood.Scientific reports · 2024Article
- Plasma-Free Blood as a Potential Alternative to Whole Blood for Transcriptomic Analysis.Phenomics (Cham, Switzerland) · 2024Article
- Ionizing radiation responses appear incidental to desiccation responses in the bdelloid rotifer Adineta vaga.BMC biology · 2024Article
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- Blood-Based mRNA Tests as Emerging Diagnostic Tools for Personalised Medicine in Breast Cancer.Cancers · 2023Review
- Whole blood RNA extraction efficiency contributes to variability in RNA sequencing data sets.PloS one · 2023Article
- The Effect of Tropical Temperatures on the Quality of RNA Extracted from Stabilized Whole-Blood Samples.International journal of molecular sciences · 2022Article
- Prediction of Time-Series Transcriptomic Gene Expression Based on Long Short-Term Memory with Empirical Mode Decomposition.International journal of molecular sciences · 2022Article
- The impact of pre-freezing storage time and temperature on gene expression of blood collected in EDTA tubes.Molecular biology reports · 2022Article
- At-home blood collection and stabilization in high temperature climates usingFrontiers in digital health · 2022Article
- Impact of storage conditions on peripheral leukocytes transcriptome.Molecular biology reports · 2021Article
- Multiple freeze-thaw cycles lead to a loss of consistency in poly(A)-enriched RNA sequencing.BMC genomics · 2021Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe reliability of RNA sequencing (RNA-seq) output is affected by the quality of RNAs, which is in turn dependent on the quality of samples. Therefore, the purposes of this study were to reconsider the threshold of the RNA integrity number (RIN) and propose a simple and efficient storage scheme of blood samples for RNA-seq. PATIENTS AND
methodsThe RNAs were extracted from blood samples that were stored at different conditions and used for sequencing. The bioinformatic analyses were performed to evaluate the impact of RNA integrity and blood sample storage conditions on the gene expression analysis.
resultsOur outcomes showed that the samples with RIN values more than 5.3 scarcely affected the quantitative results of RNA-seq, and the influence of inherent cellular physiological processes on RNA-seq output could be negligible.
conclusionThe blood samples stored at 4°C within 7 days with RIN values more than 5.3 were available for RNA-seq.
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