ArticleScientific reports2018
Automated detection and manipulation of sleep in C. elegans reveals depolarization of a sleep-active neuron during mechanical stimulation-induced sleep deprivation.
Article in Scientific reports, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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10 citing papers in PubMed, 26 citations in OpenAlex.
- The neuropeptide FLP-11 induces and self-inhibits sleep through the receptor DMSR-1 in Caenorhabiditis elegans.Current biology : CB · 2025Article
- Structural neuroplasticity after sleep loss modifies behavior and requires neurexin and neuroligin.iScience · 2024Article
- The impact of sleep problems on cerebral aneurysm risk is mediated by hypertension: a mediated Mendelian randomization study.Frontiers in genetics · 2024Article
- A sleep-active neuron can promote survival while sleep behavior is disturbed.PLoS genetics · 2023Article
- Synaptic dysfunction connects autism spectrum disorder and sleep disturbances: A perspective from studies in model organisms.Sleep medicine reviews · 2022Review
- Sleep Analysis in AdultThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- A quiescent state following mild sensory arousal in Caenorhabditis elegans is potentiated by stress.Scientific reports · 2020Article
- A microfluidic-induced C. elegans sleep state.Nature communications · 2019Article
- A GABAergic and peptidergic sleep neuron as a locomotion stop neuron with compartmentalized Ca2+ dynamics.Nature communications · 2019Article
- Sleep Counteracts Aging Phenotypes to Survive Starvation-Induced Developmental Arrest in C. elegans.Current biology : CB · 2018Article
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2 authors at 1 institution in 1 country.
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Abstract
Across species, sleep is characterized by a complex architecture. Sleep deprivation is a classic method to study the consequences of sleep loss, which include alterations in the activity of sleep circuits and detrimental consequences on well being. Automating the observation and manipulation of sleep is advantageous to study its regulation and functions. Caenorhabditis elegans shows sleep behavior similar to other animals that have a nervous system. However, a method for real-time automatic sleep detection that allows sleep-specific manipulations has not been established for this model animal. Also, our understanding of how sleep deprivation affects sleep neurons in this system is incomplete. Here we describe a system for real-time automatic sleep detection of C. elegans grown in microfluidic devices based on a frame-subtraction algorithm using a dynamic threshold. As proof of principle for this setup, we used automated mechanical stimulation to perturb sleep behavior and followed the activity of the sleep-active RIS neuron. We show that our system can automatically detect sleep bouts and deprive worms of sleep. We found that mechanical stimulation generally leads to the activation of the sleep-active RIS neuron, and this stimulation-induced RIS depolarization is most prominent during sleep deprivation.
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