ArticleNature communications2018
A promiscuous cytochrome P450 aromatic O-demethylase for lignin bioconversion.
Article in Nature communications, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
What it found
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Who cites it
81 citing papers in PubMed, 228 citations in OpenAlex.
- Structural basis for oxidative decarboxylation of lignin-derived aromatics by the fungal flavoprotein monooxygenase PcMNX1.The Journal of biological chemistry · 2026Article
- Microbial Lignin Valorization to Protocatechuic Acid and Catechol: Biofunneling Pathways and Metabolic Engineering Strategies.Biomolecules · 2026Review
- Discovery of pseudobaptigenin synthase in fungal-infected Trifolium pratense roots.The Plant journal : for cell and molecular biology · 2026Article
- Engineering Enantiocomplementary Protoglobins for Stereoconvergent Construction ofJournal of the American Chemical Society · 2026Article
- Discovery of a Structurally Distinct Acetylenase in the Biosynthesis of Mangotoxin.Journal of the American Chemical Society · 2026Article
- Characterization of acetovanillone degradation in wild-type and engineeredApplied and environmental microbiology · 2026Article
- Whole-genomic and transcriptomic analyses elucidateApplied and environmental microbiology · 2026Article
- Biochemical parallels between catabolic pathways for lignin-associated aromatic dimers.Applied and environmental microbiology · 2026Review
- A dominant subgroup of marine Bathyarchaeia assimilates organic and inorganic carbon into unconventional membrane lipids.Nature microbiology · 2025Article
- MarK, a Novosphingobium aromaticivorans kinase required for catabolism of multiple aromatic monomers.The Journal of biological chemistry · 2025Article
- Cross-kingdom comparative genomics reveal the metabolic potential of fungi for lignin turnover in deadwood.Nature ecology & evolution · 2025Article
- GHCYP706A7 governs anthocyanin biosynthesis to mitigate ROS under alkali stress in cotton.Plant cell reports · 2025Article
- Structural insights into S-lignin O-demethylation via a rare class of heme peroxygenase enzymes.Nature communications · 2025Article
- Lignin Unlocks Stealth Carbon Sinks in Cold Seeps via Microbial Enzymatic Gatekeeping.Research (Washington, D.C.) · 2025Article
- Unlocking the Healing Potential: A Comprehensive Review of Ecology and Biology of Medical-Grade Honey in Wound Management and Tissue Regeneration.Health science reports · 2025Article
- Article
- Metabolic mechanism of lignin-derived aromatics in white-rot fungi.Applied microbiology and biotechnology · 2024Review
- Characterization of a cytochrome P450 that catalyzes the O-demethylation of lignin-derived benzoates.The Journal of biological chemistry · 2024Article
- Lignin valorization to bioplastics with an aromatic hub metabolite-based autoregulation system.Nature communications · 2024Article
- Resolving the metabolism of monolignols and other lignin-related aromatic compounds in Xanthomonas citri.Nature communications · 2024Article
21 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
14 authors at 6 institutions in 3 countries.
Funding
Abstract
Microbial aromatic catabolism offers a promising approach to convert lignin, a vast source of renewable carbon, into useful products. Aryl-O-demethylation is an essential biochemical reaction to ultimately catabolize coniferyl and sinapyl lignin-derived aromatic compounds, and is often a key bottleneck for both native and engineered bioconversion pathways. Here, we report the comprehensive characterization of a promiscuous P450 aryl-O-demethylase, consisting of a cytochrome P450 protein from the family CYP255A (GcoA) and a three-domain reductase (GcoB) that together represent a new two-component P450 class. Though originally described as converting guaiacol to catechol, we show that this system efficiently demethylates both guaiacol and an unexpectedly wide variety of lignin-relevant monomers. Structural, biochemical, and computational studies of this novel two-component system elucidate the mechanism of its broad substrate specificity, presenting it as a new tool for a critical step in biological lignin conversion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.