Evidence map›Paper›PMID 29948216›Full record

Trial reportEuropean journal of nutrition2019

Gene expression changes by high-polyphenols cocoa powder intake: a randomized crossover clinical study.

P K Barrera-Reyes, N Hernández-Ramírez, J Cortés, L Poquet, K Redeuil, C Rangel-Escareño, M Kussmann, I Silva-Zolezzi, M E Tejero

Abstract readRandomized Controlled Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in European journal of nutrition, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Trial
  3. Review
  4. Review
  5. Article
  6. Review
  7. Review
  8. Review
  9. Foods (Basel, Switzerland) · 2022
    Review
  10. Review
  11. Review
  12. The Effects ofNutrients · 2020
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

P K Barrera-ReyesNutrigenomics and Nutrigenetics, National Institute of Genomic Medicine, 14610, Mexico City, Mexico.
N Hernández-RamírezNutrigenomics and Nutrigenetics, National Institute of Genomic Medicine, 14610, Mexico City, Mexico.
J CortésNutrigenomics and Nutrigenetics, National Institute of Genomic Medicine, 14610, Mexico City, Mexico.
L PoquetVitamins and Phytonutrients, Nestlé Research Centre, 1000, Lausanne, Switzerland.
K RedeuilVitamins and Phytonutrients, Nestlé Research Centre, 1000, Lausanne, Switzerland.
C Rangel-EscareñoComputational Genomics, National Institute of Genomic Medicine, 14610, Mexico City, Mexico.
M KussmannSystems Nutrition, Metabonomics and Proteomics, Nestlé Institute of Health Sciences, 1015, Lausanne, Switzerland.
I Silva-ZolezziMetabolic Programming, Nestlé Research Centre, 1000, Lausanne, Switzerland.
M E TejeroNutrigenomics and Nutrigenetics, National Institute of Genomic Medicine, 14610, Mexico City, Mexico. etejero@inmegen.gob.mx.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeTo assess the effect of the intake of a single dose of high-polyphenols cocoa on gene expression in peripheral mononuclear cells (PBMCs), and analyze conjugated (-)-epicatechin metabolites in plasma, which may be related with an antioxidant response in healthy human.

methodsA randomized, controlled, double-blind, cross-over, clinical trial in healthy young adults who consumed a single dose of high-polyphenols cocoa powder and maltodextrins as control, with a one-week washout period. Analysis of circulating metabolites, plasma antioxidant capacity and gene expression changes in PBMCs were performed under fasting conditions and 2-h after treatment using microarray in a subsample. Pathway analysis was conducted using Ingenuity Pathway Analysis (IPA).

resultsTwenty healthy participants (9 F) were included in the study. A significant increase in circulating (-)-epicatechin metabolites was found after cocoa intake in all participants without related changes in antioxidant capacity of plasma. The metabolites profile slightly varied across subjects. Treatments triggered different transcriptional changes in PBMC. A group of 98 genes showed changes in expression after cocoa treatment, while only 18 were modified by control. Differentially expressed genes included inflammatory cytokines and other molecules involved in redox balance. Gene and network analysis after cocoa intake converged in functions annotated as decreased production of reactive oxygen species (p = 9.58E-04), decreased leukocyte activation (p = 4E-03) and calcium mobilization (p = 2.51E-05).

conclusionsNo association was found between conjugated metabolites in plasma and antioxidant capacity. Changes in PBMCs gene expression suggest anti-inflammatory effects.

Indexed as

CacaoAdultAntigens, Tumor-Associated, CarbohydrateCross-Over StudiesDouble-Blind MethodFemaleGene ExpressionHumansMalePolyphenolsReference ValuesAntigens, Tumor-Associated, CarbohydratePolyphenolsAntioxidantCatechin(−)-EpicatechinFlavanolMicroarrayMolecularPBMCsROS

Identifiers

PMID29948216
PMCPMC6647247

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.