Evidence map›Paper›PMID 29948131›Full record

ArticleHeart and vessels2018

Subclinical atherosclerosis and vascular stiffness in premenopausal women: association with NOS3 and CYBA polymorphisms.

Irene Lambrinoudaki, Panagiota Chatzivasileiou, Stefanos Stergiotis, Eleni Armeni, Dimitrios Rizos, George Kaparos, Areti Augoulea, Andreas Alexandrou, Georgios Georgiopoulos, Aggeliki Laina and 1 more

Abstract read
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Article in Heart and vessels, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Irene Lambrinoudaki2nd Department of Obstetrics and Gynecology, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Str, PO Box 11528, Athens, Greece. ilambrinoudaki@med.uoa.gr.
Panagiota Chatzivasileiou2nd Department of Obstetrics and Gynecology, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Str, PO Box 11528, Athens, Greece.
Stefanos Stergiotis2nd Department of Obstetrics and Gynecology, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Str, PO Box 11528, Athens, Greece.
Eleni Armeni2nd Department of Obstetrics and Gynecology, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Str, PO Box 11528, Athens, Greece.
Dimitrios RizosHormonal and Biochemical Laboratory, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Str, PO Box 11528, Athens, Greece.
George KaparosHormonal and Biochemical Laboratory, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Str, PO Box 11528, Athens, Greece.
Areti Augoulea2nd Department of Obstetrics and Gynecology, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Str, PO Box 11528, Athens, Greece.
Andreas Alexandrou2nd Department of Obstetrics and Gynecology, Aretaieio Hospital, National and Kapodistrian University of Athens, 76 Vas. Sofias Str, PO Box 11528, Athens, Greece.
Georgios GeorgiopoulosVascular Laboratory, Department of Clinical Therapeutics, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 80 Vas. Sofias Str, PO Box 11528, Athens, Greece.
Aggeliki LainaVascular Laboratory, Department of Clinical Therapeutics, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 80 Vas. Sofias Str, PO Box 11528, Athens, Greece.
Kimon StamatelopoulosVascular Laboratory, Department of Clinical Therapeutics, Alexandra Hospital, Medical School, National and Kapodistrian University of Athens, 80 Vas. Sofias Str, PO Box 11528, Athens, Greece.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic variations of genes encoding the endothelial nitric oxide synthase (eNOS) and the NADH/NADPH oxidase system are related with atherosclerosis in the general population, but their significance in women is not sufficiently assessed. We investigated the potential association between the G894T polymorphism of the NOS3 gene and the C242T polymorphism of the CYBA gene with subclinical vascular disease. Seventy (70) healthy, normally ovulating, premenopausal women were recruited for this study. Venous blood samples were obtained for biochemical/hormonal assessment as well as for genotyping, using real-time PCR. Sonographically assessed indices of vascular structure and function included carotid and femoral intima-media thickness (IMT), flow-mediated dilation (FMD), carotid-femoral pulse-wave velocity (PWV), and augmentation index. The prevalence of wild type, heterozygote, and homozygote genotype was 44.3% (31/70), 54.3% (38/70), and 1.4% (1/70) for the G894T polymorphism and 38.6% (27/70), 31.4% (22/70), and 30.0% (21/70) for the C242T polymorphism, respectively. After multivariable adjustment, the hC242T polymorphism was a predictor of both internal carotid IMT (b-coefficient - 0.119, p = 0.011) and combined-IMT (b-coefficient - 0.061, p = 0.015). Systolic blood pressure, lipids, and hC242T determined values of FMD (b-coefficient - 1.604, p = 0.034). Concerning the NOS3 G894T polymorphism, carriers of the polymorphic variant had higher values of IMT and PWV compared to the wild-type subgroup (carotid bulb-IMT and PWV, heterozygotes/homozygotes vs wild type 0.7 ± 0.2 vs 0.6 ± 0.1 mm; 7.1 ± 0.8 vs 6.6 ± 0.7 m/s; p = 0.048 and p = 0.029, respectively). These differences, however, were rendered non-significant in the multivariable analysis. In healthy premenopausal women, the CYBA C242T polymorphism is an independent determinant of endothelial function and subclinical atherosclerosis of the carotid arteries. The NOS3 G894T polymorphic variant also correlated with atherosclerosis, an association probably mediated by the traditional risk factors for CVD. The relevance of these findings in the clinical setting remains to be elucidated.

Indexed as

Polymorphism, GeneticPremenopauseAdultAtherosclerosisCarotid ArteriesDNAFemaleGenotypeHumansNADPH OxidasesNitric Oxide Synthase Type IIIReal-Time Polymerase Chain ReactionRegional Blood FlowRisk FactorsUltrasonographyVascular StiffnessCYBA protein, humanDNANADPH OxidasesNitric Oxide Synthase Type IIINOS3 protein, humanCYBA C242T polymorphismNOS3 G894T polymorphismPremenopausal womenSubclinical atherosclerosisVascular stiffness

Identifiers

PMID29948131

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.