ArticlePlanta2018
A new high-throughput assay for determining soluble sugar in sorghum internode-extracted juice.
Article in Planta, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Effect ofFood chemistry: X · 2025Article
- Article
- Abiotic stress responses in forage crops and grasses: the role of secondary metabolites and biotechnological interventions.Frontiers in plant science · 2025Review
- Characterization and Transcriptomic Analysis of Sorghum EIN/EIL Family and Identification of Their Roles in Internode Maturation.Plants (Basel, Switzerland) · 2024Article
- Potential Roles of the GRF Transcription Factors in Sorghum Internodes during Post-Reproductive Stages.Plants (Basel, Switzerland) · 2024Article
- Harnessing the Genetic Basis of Sorghum Biomass-Related Traits to Facilitate Bioenergy Applications.International journal of molecular sciences · 2023Article
- Ginsenosides and Polysaccharides from Ginseng Co-Fermented with Multi-Enzyme-Coupling Probiotics Improve In Vivo Immunomodulatory Effects.Nutrients · 2023Article
- Editorial: Advances in crop biomass production based on multi-omics approach.Frontiers in plant science · 2023Article
- OMICS in Fodder Crops: Applications, Challenges, and Prospects.Current issues in molecular biology · 2022Review
- Using electrical impedance tomography for rapid determination of starch and soluble sugar contents in Rosa hybrida.Scientific reports · 2021Article
- Profiling Alternative 3' Untranslated Regions in Sorghum using RNA-seq Data.Frontiers in genetics · 2020Article
- Transcriptome and metabolome reveal distinct carbon allocation patterns during internode sugar accumulation in different sorghum genotypes.Plant biotechnology journal · 2019Article
- Common metabolic networks contribute to carbon sink strength of sorghum internodes: implications for bioenergy improvement.Biotechnology for biofuels · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionsA high-throughput method combining liquid handling system and 96-well microplate pipetting format was developed for total sugar determination. With this new method, we characterized diverse sugar accumulation in sorghum varieties. Sweet sorghum accumulates large amounts of sucrose in its stalk and, therefore, has emerged as one important bioenergy crop. The commonly used sugar measurement, Brix, limits the characterization of internode variation of the sugar concentrations due to its low throughput. Here we developed a low-cost, high-throughput method to determine profiles of total sugars in sorghum internodes with a liquid handling system-based sample preparation and a phenol-sulfuric acid assay in 96-well microplate format. The present method generates results highly correlated with commonly used Brix measurements (r = 0.922). The inter-assay coefficient of variation ranged from 4.8 to 7.6%. The present method can reliably estimate mixed sugars composed of 80% sucrose. We characterized the profiles of 35 sorghum accessions and identified 21 accessions with significantly different sugar concentrations between internodes either due to dried-up internodes or concentration differences. As a high-throughput alternative to Brix measurements, the new method makes it possible to phenotype total sugars from large numbers of internode samples and, therefore, will be useful for genetic and breeding purposes.
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