Evidence map›Paper›PMID 29947686›Full record

ArticleArquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery2018

THE M-RNA, EXPRESSION OF SERCA2 AND NCX1 IN THE PROCESS OF PHARMACOLOGICAL CELL PROTECTION IN EXPERIMENTAL ACUTE PANCREATITIS INDUCED BY TAUROCHOLATE.

Enio Rodrigues Vasques, José Eduardo Monteiro Cunha, Marcia Saldanha Kubrusly, Ana Maria Coelho, Sandra N Sanpietri, Helena B Nader, Ivarne L S Tersariol, Marcelo A Lima, Eleazar Chaib, Luiz Augusto Carneiro D'Albuquerque

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In one paragraph

Article in Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery, 2018. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Roles of NaWorld journal of gastroenterology · 2019
    Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Enio Rodrigues VasquesGastroenterology Department LIM 37, University of São Paulo Medical School.
José Eduardo Monteiro CunhaGastroenterology Department LIM 37, University of São Paulo Medical School.
Marcia Saldanha KubruslyGastroenterology Department LIM 37, University of São Paulo Medical School.
Ana Maria CoelhoGastroenterology Department LIM 37, University of São Paulo Medical School.
Sandra N SanpietriGastroenterology Department LIM 37, University of São Paulo Medical School.
Helena B NaderPharmacology Department of Federal University of São Paulo, São Paulo, SP, Brazil.
Ivarne L S TersariolPharmacology Department of Federal University of São Paulo, São Paulo, SP, Brazil.
Marcelo A LimaPharmacology Department of Federal University of São Paulo, São Paulo, SP, Brazil.
Eleazar ChaibGastroenterology Department LIM 37, University of São Paulo Medical School.
Luiz Augusto Carneiro D'AlbuquerqueGastroenterology Department LIM 37, University of São Paulo Medical School.
Universidade de São Paulo · BRUniversidade Federal de São Paulo · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIntracellular calcium overload is known to be a precipitating factor of pancreatic cell injury in acute pancreatitis (AP). Intracellular calcium homeostasis depends of Plasmatic Membrane Calcium ATPase (PMCA), Sarcoplasmic Endothelial Reticulum Calcium ATPase 2 (SERCA 2) and the Sodium Calcium Exchanger (NCX1). The antioxidant melatonin (Mel) and Trisulfate Disaccharide (TD) that accelerates NCX1 action could reduce the cell damage determined by the AP.

aimTo evaluate m-RNA expressions of SERCA2 and NCX1 in acute pancreatitis induced by sodium taurocholate in Wistar rats pre-treated with melatonin and/or TD.

methodsWistar rats were divided in groups: 1) without AP; 2) AP without pre-treatment; 3) AP and Melatonin; 4) AP and TD; 5) AP and Melatonin associated to TD. Pancreatic tissue samples were collected for detection of SERCA2 and NCX1 m-R NA levels by polymerase chain reaction (PCR).

resultsIncreased m-RNA expression of SERCA2 in the melatonin treated group, without increase of m-RNA expression of the NCX1. The TD did not affect levels of SERCA2 and NCX1 m-RNA expressions. The combined melatonin and TD treatment reduced the m-RNA expression of SERCA2.

conclusionsThe effect of melatonin is restricted to increased m-RNA expression of SERCA2. Although TD does not affect gene expression, its action in accelerating calcium exchanger function can explain the slightest expression of SERCA2 m-RNA when associated with Melatonin, perhaps by a joint action of drugs with different and but possibly complementary mechanisms.

Indexed as

Acute DiseaseAnimalsCytoprotectionDisaccharidesDisease Models, AnimalMaleMelatoninPancreatitisRatsRats, WistarRNA, MessengerSarcoplasmic Reticulum Calcium-Transporting ATPasesSodium-Calcium ExchangerTaurocholic AcidAtp2a2 protein, ratDisaccharidesMelatoninRNA, MessengerSarcoplasmic Reticulum Calcium-Transporting ATPasesSodium-Calcium Exchangersodium-calcium exchanger 1Taurocholic Acid

Identifiers

PMID29947686
PMCPMC6049993
OpenAlexW2811202385

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.