ArticleInhalation toxicology
Acute inhalation of ozone induces DNA methylation of apelin in lungs of Long-Evans rats.
Article in Inhalation toxicology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Ambient Air Pollution and Congenital Heart Disease: Updated Evidence and Future Challenges.Antioxidants (Basel, Switzerland) · 2025Review
- Skeletal Muscle as Endocrine Organ.Advances in experimental medicine and biology · 2025Review
- Sex Differences in Impacts of Early Gestational and Peri-Adolescent Ozone Exposure on Lung Development in Rats: Implications for Later Life Disease in Humans.The American journal of pathology · 2024Article
- Traffic-Related Air Pollution and Ground-Level Ozone Associated Global DNA Hypomethylation and Bulky DNA Adduct Formation.International journal of molecular sciences · 2023Article
- High-dimensional randomization-based inference capitalizing on classical design and modern computing.Behaviormetrika · 2023Article
- Association between Household Solid Fuel Use and Sarcopenia Risk among Middle-Aged and Older Adults in China: A Nationwide Population-Based Cohort Study.The journal of nutrition, health & aging · 2023Article
- Aging, longevity, and the role of environmental stressors: a focus on wildfire smoke and air quality.Frontiers in toxicology · 2023Review
- Impact of Exercise and Aging on Mitochondrial Homeostasis in Skeletal Muscle: Roles of ROS and Epigenetics.Cells · 2022Review
- Air pollution-induced epigenetic changes: disease development and a possible link with hypersensitivity pneumonitis.Environmental science and pollution research international · 2021Review
- Apelin/APJ system: an emerging therapeutic target for respiratory diseases.Cellular and molecular life sciences : CMLS · 2020Review
- Environmental Disinfection of a Dental Clinic during the Covid-19 Pandemic: A Narrative Insight.BioMed research international · 2020Review
- Fetal growth outcomes following peri-implantation exposure of Long-Evans rats to noise and ozone differ by sex.Biology of sex differences · 2019Article
- Ozone Exposure During Implantation Increases Serum Bioactivity in HTR-8/SVneo Trophoblasts.Toxicological sciences : an official journal of the Society of Toxicology · 2019Article
- Beta-2 Adrenergic and Glucocorticoid Receptor Agonists Modulate Ozone-Induced Pulmonary Protein Leakage and Inflammation in Healthy and Adrenalectomized Rats.Toxicological sciences : an official journal of the Society of Toxicology · 2018Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Apelin has cardiopulmonary protective properties that promote vasodilation and maintenance of the endothelial barrier. While reductions in apelin have been identified as a contributor to various lung diseases, including pulmonary edema, its role in the effect of air pollutants has not been examined. Thus, in the current study, we sought to investigate if apelin is a downstream target of inhaled ozone and if such change in expression is related to altered DNA methylation in the lung. Male, Long-Evans rats were exposed to filtered air or 1.0 ppm ozone for 4 h. Ventilation changes were assessed using whole-body plethysmography immediately following exposure, and markers of pulmonary edema and inflammation were assessed in the bronchoaveolar lavage (BAL) fluid. The enzymatic regulators of DNA methylation were measured in the lung, along with methylation and hydroxymethylation of the apelin promoter. Data showed that ozone exposure was associated with increased enhanced pause and protein leakage in the BAL fluid. Ozone exposure reduced DNA cytosine-5-methyltransferase (DNMT) activity and Dnmt3a/b gene expression. Exposure-induced upregulation of proliferating cell nuclear antigen, indicative of DNA damage, repair, and maintenance methylation. Increased methylation and reduced hydroxymethylation were measured on the apelin promoter. These epigenetic modifications accompanied ozone-induced reduction of apelin expression and development of pulmonary edema. In conclusion, epigenetic regulation, specifically increased methylation of the apelin promoter downstream of DNA damage, may lead to reductions in protective signaling of the apelinergic system, contributing to the pulmonary edema observed following the exposure to oxidant air pollution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.